Recombinant Human SPRY domain-containing SOCS box protein 2 (SPSB2)

Code CSB-YP859516HU
MSDS
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Source Yeast
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Code CSB-EP859516HU
MSDS
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Source E.coli
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Code CSB-EP859516HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859516HU
MSDS
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Source Baculovirus
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Code CSB-MP859516HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPSB2
Uniprot No.
Alternative Names
FLJ17395; Gene-rich cluster protein C9; GRCC9; MGC2519; OTTHUMP00000225007; OTTHUMP00000225008; splA/ryanodine receptor domain and SOCS box containing 2; SPRY domain containing SOCS box protein 2; SPRY domain containing SOCS box protein SSB 2; SPRY domain-containing SOCS box protein 2; Spsb2; SPSB2_HUMAN; SSB-2; SSB2
Species
Homo sapiens (Human)
Expression Region
1-263
Target Protein Sequence
MGQTALAGGS SSTPTPQALY PDLSCPEGLE ELLSAPPPDL GAQRRHGWNP KDCSENIEVK EGGLYFERRP VAQSTDGARG KRGYSRGLHA WEISWPLEQR GTHAVVGVAT ALAPLQTDHY AALLGSNSES WGWDIGRGKL YHQSKGPGAP QYPAGTQGEQ LEVPERLLVV LDMEEGTLGY AIGGTYLGPA FRGLKGRTLY PAVSAVWGQC QVRIRYLGER RAEPHSLLHL SRLCVRHNLG DTRLGQVSAL PLPPAMKRYL LYQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2. Acts as a bridge which links NOS2 with the ECS E3 ubiquitin ligase complex components ELOC and CUL5.
Gene References into Functions
  1. crystal structure of SPSB2 in complex with cyclic peptide inhibitor cR8, at a resolution of 1.34 A PMID: 28549582
  2. Shows that this gene is associated with the regulation of cell proliferation in canine mammary cancer cell lines. PMID: 19609018
Subcellular Location
Cytoplasm. Cytoplasm, cytosol.
Protein Families
SPSB family
Database Links

HGNC: 29522

OMIM: 611658

KEGG: hsa:84727

STRING: 9606.ENSP00000428338

UniGene: Hs.479856

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