Recombinant Human STAM-binding protein (STAMBP)

Code CSB-YP022794HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP022794HU
MSDS
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Source E.coli
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Code CSB-EP022794HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022794HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP022794HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
AMSH; Associated molecule with the SH3 domain of STAM; Endosome associated ubiquitin isopeptidase; Endosome-associated ubiquitin isopeptidase; MGC126516; MGC126518; MICCAP; STABP_HUMAN; STAM binding protein; STAM-binding protein; Stambp
Species
Homo sapiens (Human)
Expression Region
1-424
Target Protein Sequence
MSDHGDVSLP PEDRVRALSQ LGSAVEVNED IPPRRYFRSG VEIIRMASIY SEEGNIEHAF ILYNKYITLF IEKLPKHRDY KSAVIPEKKD TVKKLKEIAF PKAEELKAEL LKRYTKEYTE YNEEKKKEAE ELARNMAIQQ ELEKEKQRVA QQKQQQLEQE QFHAFEEMIR NQELEKERLK IVQEFGKVDP GLGGPLVPDL EKPSLDVFPT LTVSSIQPSD CHTTVRPAKP PVVDRSLKPG ALSNSESIPT IDGLRHVVVP GRLCPQFLQL ASANTARGVE TCGILCGKLM RNEFTITHVL IPKQSAGSDY CNTENEEELF LIQDQQGLIT LGWIHTHPTQ TAFLSSVDLH THCSYQMMLP ESVAIVCSPK FQETGFFKLT DHGLEEISSC RQKGFHPHSK DPPLFCSCSH VTVVDRAVTI TDLR
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes. Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization. Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways.
Gene References into Functions
  1. Contrary to previously reported STAMBP mutations, the Ser236Phe mutation did not lead to constitutive activation of the PI3K-AKT-mTOR pathway in patient-derived LCLs, as indicated by the expression of phosphorylated S6 ribosomal protein, suggesting that it is not the major pathomechanism underlying the disorder in this patient. PMID: 29907875
  2. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity. PMID: 28492230
  3. Two siblings with classic features of MIC-CAP syndrome that harbor a novel predicted splice mutation in STAMBP PMID: 25692795
  4. associated molecule with the SH3 domain of STAM-mediated deubiquitination of connexin 43 protects Gap junctions from degradation PMID: 25070368
  5. Recognition of the proximal ubiquitin is imperative for the linkage specificity and catalytic efficiency of AMSH. PMID: 24151880
  6. Mutations in STAMBP, encoding a deubiquitinating enzyme, cause microcephaly-capillary malformation syndrome. PMID: 23542699
  7. AMSH efficiently removes ubiquitin from the activated EGFR. PMID: 22800866
  8. tight coupling of ESCRT-III CHMP3 and AMSH functions and provide insight into the regulation of ESCRT-III PMID: 21827950
  9. Studies indicate that USP8/Ubpy and AMSH interact with ESCRT components to modulate the ubiquitination status of receptors and relevant sorting proteins. PMID: 21448666
  10. AMSH interacts with ESCRT-0 to regulate the stability and trafficking of CXCR4. PMID: 20159979
  11. AMSH is a deubiquitinating enzyme with functions at the endosome to oppose ubiquitin-dependent receptor sorting PMID: 15314065
  12. The cellular functions of UBPY are complex but clearly distinct from those of the Lys-63-ubiquitin-specific protease, AMSH, with which it shares a binding site on the SH3 domain of STAM PMID: 16520378
  13. AMSH and AMSH-LP are anchored on the early endosomal membrane via interaction with the clathrin coat PMID: 16716190
  14. AMSH has a role in the deubiquitination of the endosomal cargo preceding lysosomal degradation PMID: 16760479
  15. AMSH, which has been recently characterized as a regulator of the endosomal sorting of epidermal growth factor receptor, represents a novel modulator of CaR signaling. PMID: 16854379
  16. both the deubiquitination enzyme activity of AMSH and its CHMP3-binding ability are required to clear ubiquitinated cargo from endosomes PMID: 17159328
  17. Endosomal AMSH (STAMBP) is a functional component of the multivesicular body pathway. PMID: 17261583
  18. UBPY MIT domain and another ubiquitin isopeptidase, AMSH, reveals common interactions with CHMP1A and CHMP1B but a distinct selectivity of AMSH for CHMP3/VPS24, a core subunit of the ESCRT-III complex, and UBPY for CHMP7. PMID: 17711858
  19. crystal structures of the human AMSH-LP DUB domain alone and in complex with a Lys 63-linked di-ubiquitin at 1.2 A and 1.6 A resolutions, respectively PMID: 18758443
  20. Data suggest that AMSH and UBPY are essential for trafficking and down-regulation of PAR(2) but not for regulating PAR(2) dissociation from beta-arrestin2 or PAR(2)-mediated ERK2 activation. PMID: 19684015

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Involvement in disease
Microcephaly-capillary malformation syndrome (MICCAP)
Subcellular Location
Nucleus. Membrane; Peripheral membrane protein. Cytoplasm. Early endosome.
Protein Families
Peptidase M67C family
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 16950

OMIM: 606247

KEGG: hsa:10617

STRING: 9606.ENSP00000344742

UniGene: Hs.469018

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