Recombinant Human Serine/threonine-protein kinase 38 (STK38)

Code CSB-YP614885HU
MSDS
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Source Yeast
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Code CSB-EP614885HU
MSDS
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Source E.coli
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Code CSB-EP614885HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP614885HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP614885HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
STK38
Uniprot No.
Alternative Names
NDR 1; NDR; Ndr Ser/Thr kinase-like protein; NDR1; NDR1 protein kinase; Nuclear Dbf2 related kinase 1; nuclear Dbf2-related 1; Nuclear Dbf2-related kinase 1; Nuclear Dbf2-related protein; Serine/threonine protein kinase 38; Serine/threonine-protein kinase 38; STK 38; STK38; STK38_HUMAN
Species
Homo sapiens (Human)
Expression Region
2-465
Target Protein Sequence
AMTGSTPCS SMSNHTKERV TMTKVTLENF YSNLIAQHEE REMRQKKLEK VMEEEGLKDE EKRLRRSAHA RKETEFLRLK RTRLGLEDFE SLKVIGRGAF GEVRLVQKKD TGHVYAMKIL RKADMLEKEQ VGHIRAERDI LVEADSLWVV KMFYSFQDKL NLYLIMEFLP GGDMMTLLMK KDTLTEEETQ FYIAETVLAI DSIHQLGFIH RDIKPDNLLL DSKGHVKLSD FGLCTGLKKA HRTEFYRNLN HSLPSDFTFQ NMNSKRKAET WKRNRRQLAF STVGTPDYIA PEVFMQTGYN KLCDWWSLGV IMYEMLIGYP PFCSETPQET YKKVMNWKET LTFPPEVPIS EKAKDLILRF CCEWEHRIGA PGVEEIKSNS FFEGVDWEHI RERPAAISIE IKSIDDTSNF DEFPESDILK PTVATSNHPE TDYKNKDWVF INYTYKRFEG LTARGAIPSY MKAAK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2.
Gene References into Functions
  1. Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop. PMID: 30018336
  2. Study provides evidence for an inhibitory role of SOCS2 in TNFalpha induced NF-kappa B activation, identifies NDR1 as a novel substrate of SOCS2, and demonstrates that SOCS2 and TNFalpha induced NF-kappa B signaling are linked through NDR1. PMID: 28216640
  3. Data show that STK38 supports Ras-driven transformation through promoting detachment-induced autophagy. PMID: 27283898
  4. a novel and intimate link between the protein kinase NDR1 and TGFbeta signaling PMID: 23840619
  5. Data establish STK38 as a conserved regulator of autophagy in human cells and flies. PMID: 26387716
  6. PLK1-mediated phosphorylation protects the binding of Mob1 to NDR1 and subsequent NDR1 activation PMID: 26057687
  7. STK38-mediated phosphorylation of CDC25A at Ser-76 and the subsequent degradation of CDC25A are required to promote DNA damage-induced G2/M checkpoint activation. PMID: 25936524
  8. STK38 regulates MYC protein stability and turnover in a kinase activity-dependent manner. STK38 kinase inactivation abrogates apoptosis following B-cell receptor activation, whereas its silencing significantly decreases MYC levels and increases apoptosis. PMID: 23178486
  9. cyclin D1 has a role in promoting cell cycle progression by enhancing NDR1/2 kinase activity independent of Cdk4 PMID: 23897809
  10. 17-allylamino-17-demethoxygeldanamycin down-regulates STK38, modulating radiosensitivity. PMID: 23886587
  11. Findings suggest a function of NDR1, which may regulate NF-kappaB activation by its kinase activity. PMID: 22674419
  12. GSK-3beta inhibits full activation of STK38, and STK38 activation is required to prevent cell death in response to oxidative stress PMID: 22142472
  13. Findings show a biological role for MICAL-1 in apoptosis and define a novel negative regulatory mechanism of MST-NDR signaling. PMID: 21730291
  14. These findings establish a novel MST3-NDR-p21 axis as an important regulator of G(1)/S progression of mammalian cells. PMID: 21262772
  15. Data indicate that human MOB2 is a negative regulator of human NDR kinases in biochemical and biological settings. PMID: 20624913
  16. NDR1 and NDR2 serine-threonine kinases are regulated by mob proteins PMID: 15067004
  17. an insert within the catalytic domain between subdomains VII and VIII is autoinhibitory, and the binding of human MOB1 to the N-terminal domain of NDR induces the release of this autoinhibition. PMID: 15197186
  18. The NDR1 and NDR2 kinases were incorporated into HIV-1 particles and were cleaved by the HIV-1 protease. PMID: 15582665
  19. In vivo activation of human NDR by membrane-bound hMOBs is dependent on their interaction and occurs solely at the membrane. PMID: 16135814
  20. These data indicate that NDR-driven centrosome duplication requires Cdk2 activity and that Cdk2-induced centrosome amplification is affected upon reduction of NDR activity. PMID: 17317633
  21. phosphorylation of MOB1 at Thr74 by MST2 is essential to make a complex of MOB1, MST2 and NDR1, and to fully activate NDR1 PMID: 18362890

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family
Tissue Specificity
Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Database Links

HGNC: 17847

OMIM: 606964

KEGG: hsa:11329

STRING: 9606.ENSP00000229812

UniGene: Hs.409578

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