Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

AANAT

Uniprot No.

Q16613

Research Area

Neuroscience

Alternative Names

AA-NAT; Aanat; Aralkylamine N-acetyltransferase; Arylalkylamine N acetyltransferase; Serotonin acetylase; Serotonin N-acetyltransferase; SNAT; SNAT_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-207aa

Target Protein Sequence

MSTQSTHPLKPEAPRLPPGIPESPSCQRRHTLPASEFRCLTPEDAVSAFEIEREAFISVLGVCPLYLDEIRHFLTLCPELSLGWFEEGCLVAFIIGSLWDKERLMQESLTLHRSGGHIAHLHVLAVHRAFRQQGRGPILLWRYLHHLGSQPAVRRAALMCEDALVPFYERFSFHAVGPCAITVGSLTFMELHCSLRGHPFLRRNSGC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

51.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-207 constitute the expression domain of recombinant Human AANAT. This AANAT protein is theoretically predicted to have a molecular weight of 51.8 kDa. This AANAT protein is produced using e.coli expression system. Fusion of the N-terminal 10xHis-GST tag into the AANAT encoding gene fragment was conducted, allowing for easier detection and purification of the AANAT protein in subsequent stages.

Human serotonin N-Acetyltransferase (AANAT) is a key enzyme in the synthesis of melatonin, the hormone that regulates circadian rhythms. AANAT catalyzes the conversion of serotonin to N-acetylserotonin, a precursor in melatonin biosynthesis. In chronobiology, AANAT is pivotal for the molecular clock, linking light-dark cycles to melatonin production. In neuroscience, AANAT influences sleep-wake cycles and mood regulation. In endocrinology, melatonin has roles in reproductive physiology and immune function. Investigating AANAT provides insights into circadian rhythm regulation, sleep disorders, and mood-related conditions, offering potential applications in chronotherapy, neuropsychiatry, and endocrine research for therapeutic interventions and understanding melatonin's broader physiological roles.

Target Background

Function

Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.

Gene References into Functions

Aggregate genetic variation in circadian rhythm and melatonin pathways were significantly associated with the risk of prostate cancer in data combining GAME-ON and PLCO, after Bonferroni correction (ppathway < 0.00625). The two most significant genes were NPAS2 (pgene = 0.0062) and AANAT (pgene = 0.00078); the latter being significant after Bonferroni correction. PMID: 28699174
Data show that arylalkymine N-acetyltransferase (AANAT) levels and melatonin synthesis change after transient receptor potential channel 4 (TRPV4 channel) stimulation in ciliary body epithelial cells. PMID: 28368307
these and related results indicate both a major involvement of the N-end rule pathway in the control of rodent AANATs and substantial differences in the regulation of rodent and human AANATs that stem from differences in their N-terminal sequences. PMID: 27339900
There is dysregulation of the AANAT/ASMT/melatonin --> melatonin receptor axis in cholangiocarcinoma, which inhibited melatonin secretion and subsequently enhanced CCA growth. PMID: 21778461
The expression of AANAT in epithelial cells of striated ducts in human submandibular glands. PMID: 21437622
The functional expression of human SNA protein was closely associated with the elevated synthesis of N-acetylserotonin and melatonin in transgenic rice plants. PMID: 20586889
Identified 17 sequence changes in AANAT gene of patients with major depression. Show evidence of the association of genetic variability in the AANAT gene with susceptibility to major depression. PMID: 20459461
There is a significant increase in AANAT allele positivity at the single nucleotide polymorphism (alanine 129--> threonine) at between patients with DSPS & controls. AA-NAT could be a susceptibility gene for DSPS. PMID: 12736803
Data suggest that the -263G/C single nucleotide polymorphism of arylalkylamine-N-acetyl-transferase (AA-NAT) may be an important determinant of the late/short sleep pattern. PMID: 15332344
Single nucleotide polymorphisms in the AANAT gene identified thus far cannot explain the observed interindividual differences for nocturnal melatonin profiles in the subjects investigated. PMID: 18569588
AANAT polymorphisms were not associated with adolescent idiopathic scoliosis. PMID: 18794762
Observational study of genotype prevalence and gene-disease association. (HuGE Navigator) PMID: 17503170

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Subcellular Location

Cytoplasm.

Protein Families

Acetyltransferase family, AANAT subfamily

Tissue Specificity

Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.

Database Links

HGNC: 19

OMIM: 600950

KEGG: hsa:15

STRING: 9606.ENSP00000250615

UniGene: Hs.431417

Code	CSB-EP623090HU
Abbreviation	Recombinant Human AANAT protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Serotonin N-acetyltransferase (AANAT)

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