AANAT Antibody

Code CSB-PA623090LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, 293T whole cell lysate
    All lanes: AANAT antibody at 7.5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 24, 29 kDa
    Observed band size: 29 kDa

  • Immunofluorescence staining of A549 cells with CSB-PA623090LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AANAT Polyclonal antibody
Uniprot No. Q16613
Target Names AANAT
Alternative Names AA-NAT antibody; Aanat antibody; Aralkylamine N-acetyltransferase antibody; Arylalkylamine N acetyltransferase antibody; Serotonin acetylase antibody; Serotonin N-acetyltransferase antibody; SNAT antibody; SNAT_HUMAN antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Serotonin N-acetyltransferase protein (1-207AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
Gene References into Functions
  1. Aggregate genetic variation in circadian rhythm and melatonin pathways were significantly associated with the risk of prostate cancer in data combining GAME-ON and PLCO, after Bonferroni correction (ppathway < 0.00625). The two most significant genes were NPAS2 (pgene = 0.0062) and AANAT (pgene = 0.00078); the latter being significant after Bonferroni correction. PMID: 28699174
  2. Data show that arylalkymine N-acetyltransferase (AANAT) levels and melatonin synthesis change after transient receptor potential channel 4 (TRPV4 channel) stimulation in ciliary body epithelial cells. PMID: 28368307
  3. these and related results indicate both a major involvement of the N-end rule pathway in the control of rodent AANATs and substantial differences in the regulation of rodent and human AANATs that stem from differences in their N-terminal sequences. PMID: 27339900
  4. There is dysregulation of the AANAT/ASMT/melatonin --> melatonin receptor axis in cholangiocarcinoma, which inhibited melatonin secretion and subsequently enhanced CCA growth. PMID: 21778461
  5. The expression of AANAT in epithelial cells of striated ducts in human submandibular glands. PMID: 21437622
  6. The functional expression of human SNA protein was closely associated with the elevated synthesis of N-acetylserotonin and melatonin in transgenic rice plants. PMID: 20586889
  7. Identified 17 sequence changes in AANAT gene of patients with major depression. Show evidence of the association of genetic variability in the AANAT gene with susceptibility to major depression. PMID: 20459461
  8. There is a significant increase in AANAT allele positivity at the single nucleotide polymorphism (alanine 129--> threonine) at between patients with DSPS & controls. AA-NAT could be a susceptibility gene for DSPS. PMID: 12736803
  9. Data suggest that the -263G/C single nucleotide polymorphism of arylalkylamine-N-acetyl-transferase (AA-NAT) may be an important determinant of the late/short sleep pattern. PMID: 15332344
  10. Single nucleotide polymorphisms in the AANAT gene identified thus far cannot explain the observed interindividual differences for nocturnal melatonin profiles in the subjects investigated. PMID: 18569588
  11. AANAT polymorphisms were not associated with adolescent idiopathic scoliosis. PMID: 18794762
  12. Observational study of genotype prevalence and gene-disease association. (HuGE Navigator) PMID: 17503170

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Subcellular Location Cytoplasm
Protein Families Acetyltransferase family, AANAT subfamily
Tissue Specificity Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
Database Links

HGNC: 19

OMIM: 600950

KEGG: hsa:15

STRING: 9606.ENSP00000250615

UniGene: Hs.431417

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