AANAT Antibody

Datasheet
Code CSB-PA623090LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q16613
Image
  • Western Blot
    Positive WB detected in: 293 whole cell lysate, 293T whole cell lysate
    All lanes: AANAT antibody at 7.5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 24, 29 kDa
    Observed band size: 29 kDa

  • Immunofluorescence staining of A549 cells with CSB-PA623090LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Serotonin N-acetyltransferase protein (1-207AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
Relevance Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Serotonin N-acetyltransferase (Serotonin acetylase) (EC 2.3.1.87) (Aralkylamine N-acetyltransferase) (AA-NAT), AANAT, SNAT
Immunogen Species Human
Research Area Neuroscience
Target Names AANAT
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Controls the night/day rhythm of melatonin production in the pineal gland. Catalyzes the N-acetylation of serotonin into N-acetylserotonin, the penultimate step in the synthesis of melatonin.
Subcellular Location Cytoplasm
Protein Families Acetyltransferase family, AANAT subfamily
Tissue Specificity Highly expressed in pineal gland and at lower levels in the retina. Weak expression in several brain regions and in the pituitary gland.
Database Links

HGNC: 19

OMIM: 600950

KEGG: hsa:15

STRING: 9606.ENSP00000250615

UniGene: Hs.431417

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