Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SMUG1

Uniprot No.

Q53HV7

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

FDG; HMUDG; MGC104370; Single strand selective monofunctional uracil DNA glycosylase 1; Single strand selective monofunctional uracil DNA glycosylase; Single-strand selective monofunctional uracil DNA glycosylase; SMUG 1; Smug1; SMUG1 protein; SMUG1_HUMAN; UNG 3; UNG3

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-177aa

Target Protein Sequence

MPQAFLLGSIHEPAGALMEPQPCPGSLAESFLEEELRLNAELSQLQFSEPVGIIYNPVEYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGEVSMVRDWLGIVGPVLTPPQEHPKRPVLGLECPQSEGPRQSMGHEIKSELLMGGCSWIRGKIQCDRVQVRRPGFSSQL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

35.6kDa

Protein Length

Full Length of Isoform 2

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-177 constitute the expression domain of recombinant Human SMUG1. This SMUG1 protein is expected to have a theoretical molecular weight of 35.6 kDa. Expression of this SMUG1 protein is conducted in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the SMUG1 encoding gene fragment was conducted, allowing for easier detection and purification of the SMUG1 protein in subsequent stages.

Human single-strand selective monofunctional uracil DNA glycosylase (SMUG1) is a DNA repair enzyme crucial for base excision repair. SMUG1 specifically recognizes and removes uracil from single-stranded DNA, preventing mutagenesis. In genomics, SMUG1 is essential for maintaining genome integrity and stability. Research on SMUG1 extends to immunology, where it influences somatic hypermutation during antibody maturation. Additionally, SMUG1 is implicated in neurobiology, playing a role in oxidative DNA damage repair in neurons. Investigating SMUG1 provides insights into DNA repair mechanisms, genomic stability, and disease susceptibility, offering potential applications in understanding and preventing mutagenic events, as well as implications for immunology and neurological disorders.

Target Background

Function

Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.

Gene References into Functions

This analysis showed a relative increase in the expression of E2F6 in gastric adenocarcinoma with no lymph node metastasis (chi (2), P = 0.04 and OR, P = 0.08), while overexpression of RhoA and SMUG1 was found more often in the diffuse subtype of gastric adenocarcinoma as compared to the intestinal subtype. PMID: 27909884
Our study showed that c.-31A/G-SMUG1 genotypes/alleles do not have any association with the occurrence or severity of advanced type age-related macular degeneration (AMD). There was no interaction of CRP levels and SMUG1 genotypes in AMD susceptibility. PMID: 28095127
A case-control study of 801 bladder cancer patients and 801 matched controls, the associations of 167 single nucleotide polymorphisms (SNPs) from 19 genes of the BER pathway with the risk of bladder cancer; 13 SNPs in 10 Base excision repair (BER) pathway genes were significantly associated with bladder cancer risk; most significant SNP was rs2029167 in the SMUG1 gene. PMID: 24038406
Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1) deficiency is linked to aggressive breast cancer and predicts response to adjuvant therapy. PMID: 24253812
The results obtained suggest the potential role of the g.4235T>C and the c.-31A>G polymorphisms in AMD pathogenesis. PMID: 23714858
There was no difference between SMUG1 proficient and depleted cells following continuous exposure. PMID: 23253900
SMUG1 is a DKC1 interaction partner that contributes to rRNA quality control, partly by regulating 5-hydroxymethyluridine levels. PMID: 23246433
Data show that uracil-DNA glycosylases SMUG1 and UNG2 display widely different sequence preferences. PMID: 22483865
there was increased risk of breast cancer among postmenopausal women heterozygous for either SMUG1 rs2029166 or rs7296239. Among premenopausal women, the increased risk associated with SMUG1 rs2029166 was limited to those with low folate intake. PMID: 21427733
analysis of species specific differences between mouse and humans in regulation of SMUG1 and UNG2 PMID: 21454529
hSMUG1 is a broad specificity backup for hUNG2, the major enzyme for removal of deaminated cytosine in single strnaded DNA PMID: 12161446
This enzyme has a role in repair of 5-formyluracil and other oxidized and deaminated base lesions. PMID: 12718543
The structure and specificity of SMUG1 have been solved. PMID: 12820976
Site-directed mutagenesis was used to determine the catalytic and DNA damage-recognition mechanism of hSMUG1. PMID: 15466595
SMUG1 plays little natural role in antibody diversification. PMID: 16407970
A G44T missense mutation was found in familial colorectal cancer DNA suggesting a limited role for this gene in the devlopment of CRC. PMID: 17029639
Analysis of the catalytic and precision damage recognition mechanisms of SMUG1. PMID: 17150750
SMUG1 and UNG2 coordinate the initial steps in base excision repair of U:G mismatches by different molecular mechanisms. PMID: 17537817
proline substitution at the G63 position switches the Gme SMUG1 enzyme to an exclusive UDG as demonstrated by the uniform excision of uracil in both double-stranded and single-stranded DNA and the complete loss of XDG activity PMID: 18835277
Properties used by hSMUG1 to select damaged pyrimidines include the size and free energy of solvation of the 5-substituent but not electronic inductive properties. PMID: 19324873
hSMUG1 excised fU from DNA opposite all normal bases with the highest activity when opposite non-cognate C or T followed by G and cognate A PMID: 19365746

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Subcellular Location

Nucleus.

Protein Families

Uracil-DNA glycosylase (UDG) superfamily, SMUG1 family

Database Links

HGNC: 17148

OMIM: 607753

KEGG: hsa:23583

STRING: 9606.ENSP00000338606

UniGene: Hs.632721

Code	CSB-EP706636HU
Abbreviation	Recombinant Human SMUG1 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Single-strand selective monofunctional uracil DNA glycosylase (SMUG1)

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Target Background

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