Recombinant Human Sodium- and chloride-dependent glycine transporter 2 (SLC6A5), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

SLC6A5

Uniprot No.

Alternative Names

Glycine transporter; Glycine transporter type 2; GlyT-2; GlyT2; NET1; SC6A5_HUMAN; SC6AC5; Slc6a5; SLC6A5 solute carrier family 6 neurotransmitter transporter, glycine member 5; Slc6a9; Sodium and chloride dependent glycine transporter 2; Sodium- and chloride-dependent glycine transporter 2; Solute carrier family 6 member 5; Solute carrier family 6 neurotransmitter transporter glycine member 5

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Sodium- and chloride-dependent glycine transporter. Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses.

Gene References into Functions

analysis of the human SLC6A5 gene mutation associated with hyperekplexia PMID: 25480793
Report that in the presence of a GlyT2 mechanism-based toxicity, reversible inhibitors might allow a tolerable balance between efficacy and toxicity. PMID: 23962079
Constitutive endocytosis and turnover of the neuronal glycine transporter GlyT2 is dependent on ubiquitination of a C-terminal lysine cluster. PMID: 23484054
A novel dominant hyperekplexia mutation Y705C alters trafficking and biochemical properties of the presynaptic glycine transporter GlyT2. PMID: 22753417
This study firmly establishes the combination of missense, nonsense, frameshift, and splice site mutations in the GlyT2 gene as the second major cause of startle disease. PMID: 22700964
A transgenic cell line is studied in which green fluorescent protein (GFP) is expressed under the control of the promoter for the glycine transporter GlyT2 during zebrafish development. PMID: 21397641
Inspiratory-modulated neurons with pacemaker properties are present in the preBotzinger complex of newborn transgenic mice and express the glycine tranporter (GlyT)2 protein. PMID: 20219997
Variants not associated with bipolar disorder or schizophrenia. PMID: 16691125
SLC6A5 mutations result in defective subcellular GlyT2 localization, decreased glycine uptake or both, with selected mutations affecting predicted glycine and Na+ binding sites. PMID: 16751771
results are consistent with GLYT2 being a disease gene in human hyperekplexia PMID: 16884688
SLC6A5 gene is associated with schizophrenia. PMID: 18638388

Hide All

Involvement in disease

Hyperekplexia 3 (HKPX3)

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family, SLC6A5 subfamily

Tissue Specificity

Expressed in medulla, and to a lesser extent in spinal cord and cerebellum.

Database Links

HGNC: 11051

OMIM: 604159

KEGG: hsa:9152

STRING: 9606.ENSP00000434364

UniGene: Hs.136557

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Recombinant Human Sodium- and chloride-dependent glycine transporter 2 (SLC6A5), partial

Product Details

Customer Reviews and Q&A

Target Background

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