Recombinant Human Solute carrier family 22 member 9 (SLC22A9), partial

Code CSB-YP811607HU
MSDS
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Source Yeast
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Code CSB-EP811607HU
MSDS
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Source E.coli
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Code CSB-EP811607HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP811607HU
MSDS
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Source Baculovirus
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Code CSB-MP811607HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLC22A9
Uniprot No.
Alternative Names
SLC22A9; hOAT4; OAT7; UST3; Solute carrier family 22 member 9; Organic anion transporter 7
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sodium-independent organic anion transporter which exhibits high specificity for sulfated conjugates of xenobiotics and steroid hormones. It is also specifically activated by 3 to 5 carbons-containing short-chain fatty acids/SCFAs, including propionate, butyrate and valerate. May operate the exchange of sulfated organic components against short-chain fatty acids/SCFAs at the sinusoidal membrane of hepatocytes.
Gene References into Functions
  1. two human organic anion transporters OAT2 and OAT7 activities were assessed. PMID: 28945155
  2. Pravastatin is the first identified OAT7 drug substrate. Substantial inter-individual variability in hepatic OAT7 expression, majorly driven by HNF4alpha, may contribute to pravastatin drug disposition and might affect response. PMID: 26239079
  3. uptake of steroid sulfates by isolated trophoblasts is mediated by OATP-B and OAT-4 suggesting a physiological role of both carrier proteins in placental uptake of fetal-derived steroid sulfates. PMID: 12409283
  4. Both progesterone and activation of PKC inhibited hOAT4 activity through redistribution of the transporter from cell surface to the intracellular compartments. PMID: 17341544
  5. OAT7 is the first liver-specific transporter among members of the organic anion transporters of SLC22 family. PMID: 17393504
  6. The data provide a model for the concerted action of OAT1 mediating apical secretion of glutarate derivatives from proximal tubule cells PMID: 18365245
  7. The regulatory single nucleotide polymorphism (SNP) of OAT4 found in the promoter region of nephrectomized patients is unlikely to influence mRNA expression or promoter activity of OAT4. PMID: 18414781
  8. Review summarizes current knowledge on the functional and phenotypic consequences of genetic variation in intestinally, hepatically and renally expressed members of solute carrier family (SLC22) member 9. PMID: 18466105
  9. Functional differences in steroid uptake of SLC22A9 and SLC02B1 in human placenta are reported. PMID: 18501590
  10. Human organic anion transporter hOAT4 is a transporter of perfluorooctanoic acid. PMID: 19371258

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Subcellular Location
Basolateral cell membrane; Multi-pass membrane protein. Note=Enriched at the sinusoidal part of the plasma membrane.
Protein Families
Major facilitator (TC 2.A.1) superfamily, Organic cation transporter (TC 2.A.1.19) family
Tissue Specificity
Specifically expressed in liver by hepatocytes (at protein level).
Database Links

HGNC: 16261

OMIM: 607579

KEGG: hsa:114571

STRING: 9606.ENSP00000279178

UniGene: Hs.502772

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