Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SMPD1

Uniprot No.

P17405

Research Area

Neuroscience

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

47-631aa

Target Protein Sequence

LALSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPYPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYENTLAAQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYRVYQIDGNYSGSSHVVLDHETYILNLTQANIPGAIPHWQLLYRARETYGLPNTLPTAWHNLVYRMRGDMQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPDGSLPEAQSLWPRPLFC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

78.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant human sphingomyelin phosphodiesterase (SMPD1) is generated by inserting the gene encoding the full-length mature human SMPD1 protein (47-631aa) and the N-terminal 6xHis-SUMO-tag gene into a plasmid and transferring the recombinant plasmid into E.coli cells for protein production. The recombinant gap protein is isolated and purified from the cell lysate. Its purity is greater than 90% measured by SDS-PAGE. On the gel, the recombinant SMPD1 protein appears as a band with a molecular weight of approximately 79 kDa.

SMPD1 mainly hydrolyzes sphingomyelin, a key component of cell membranes, into ceramide and phosphocholine. This enzymatic activity is essential for sphingolipid metabolism, lysosomal function, cellular signaling, and maintaining cellular homeostasis.

Target Background

Function

Converts sphingomyelin to ceramide. Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly. However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form.; In the lysosomes, converts sphingomyelin to ceramide. Plays an important role in the export of cholesterol from the intraendolysosomal membranes. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Modulates stress-induced apoptosis through the production of ceramide.; When secreted, modulates cell signaling with its ability to reorganize the plasma membrane by converting sphingomyelin to ceramide. Secreted form is increased in response to stress and inflammatory mediators such as IL1B, IFNG or TNF as well as upon infection with bacteria and viruses. Produces the release of ceramide in the outer leaflet of the plasma membrane playing a central role in host defense. Ceramide reorganizes these rafts into larger signaling platforms that are required to internalize P. aeruginosa, induce apoptosis and regulate the cytokine response in infected cells. In wounded cells, the lysosomal form is released extracellularly in the presence of Ca(2+) and promotes endocytosis and plasma membrane repair.; Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.; Lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.; (Microbial infection) Secretion is activated by bacteria such as P. aeruginos, N. gonorrhoeae and others, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection.; (Microbial infection) Secretion is activated by human coronaviruses SARS-CoV and SARS-CoV-2 as well as Zaire ebolavirus, this activation results in the release of ceramide in the outer leaflet of the plasma membrane which facilitates the infection.

Gene References into Functions

The novel p.P226Q mutation and p.P533L mutation of SMPD1 are associated with Parkinson's disease. PMID: 29896723
the human ASM holoenzyme and product bound structures encompassing all of the functional domains, are presented. PMID: 27725636
This study sheds light on the molecular mechanism of ASMase function. PMID: 27435900
The results suggest an association of Acid sphingomyelinase activation with Respiratory Syncytial Virus infection, a cause for common acute illness. PMID: 29238000
This study identified association between Leu-Ala (Val) repeat variants in SMPD1 and Chinese Han patients with sporadic Parkinson's disease. PMID: 27814975
Parkinson's disease is associated with mutations in SMPD1 gene in Ashkenazi Jews. PMID: 27449028
Through genetic analysis it was determined that genetic variants in SMPD1 increase the risk of Parkinson's Disease in the Chinese Han population. PMID: 26377108
Enzyme activities (acid alpha-glucosidase (GAA), galactocerebrosidase (GALC), glucocerebrosidase (GBA), alpha-galactosidase A (GLA), alpha-iduronidase (IDUA) and sphingomyeline phosphodiesterase-1 (SMPD-1)) were measured on ~43,000 de-identified dried blood spot (DBS) punches, and screen positive samples were submitted for DNA sequencing to obtain genotype confirmation of disease risk PMID: 27238910
This study shows that the ASM alternative splicing pattern could be a biological target with diagnostic relevance and could serve as a novel biomarker for Major depressive disorder PMID: 27866044
stress-induced activation of p38 MAPK and apoptosis in endothelial cells and established the link between the acid sphingomyelinase/ceramide and p38 MAPK pathways. PMID: 28179144
Elevated acid sphingomyelinase (ASM) activity in the lung tumor environment and blood serum of patients with non-small cell lung cancer (NSCLC). PMID: 28883000
this is the largest study on mutation analysis of patients with ASM-deficient Niemann-Pick disease reported in literature and also the first study on the SMPD1 gene mutation spectrum in India. PMID: 27338287
ASM and ceramides, together with STX6 and cholesterol, constitute a new regulatory mechanism for the exit of Met from the Golgi during its biosynthetic route. PMID: 27802163
the p.Ala359Asp mutation causes structural alterations in the hydrophobic environment where ASM is located, decreasing its enzymatic activity PMID: 27659707
Airway cells with lower SMPD1 activity increases neutrophil chemotaxis towards them. PMID: 27865842
The results identify acid sphingomyelinase as a novel target of Lycium Chinense berries to decrease saturated/unsaturated fatty acid sphingomyelin ratio, known to be useful for cell health. Consistent with these data, the berries regulate specifically gene expression to protect cells from apoptosis. PMID: 27756324
ASMase mediated the 50-Hz MF-induced EGFR clustering via ceramide which was produced from hydrolyzation on lipid rafts PMID: 26915736
acid sphingomyelinase-1 PMID: 27352097
ASM is a negative regulator of regulatory T Cell development. PMID: 27512981
We report on an infant with a new frameshift mutation (c.575dupG) of the SMPD1 gene. PMID: 26766671
Seven novel mutations (c.518-519insT, c.562_563insC, c.792Gdel, c.949G>A, c.1487_1499delACCGTGTGTACCA, c.1495T>C and c.1670T>C) of the SMPD1 gene were identified in four patients. Only one fetus had two mutations of the SMPD1 gene of amniocytes PMID: 26851525
although ebeta,5alpha,6beta-triol is more specific than 7-KC, it has been found to be significantly elevated in patients with Niemann-Pick type A, a lysosomal storage disorder caused by mutations in the SMPD1 gene PMID: 26790753
This work provides the first evidence of significantly elevated circulating secretory sphyngomyelinase activity in the first trimester of pregnancy in women who go on to develop preeclampsia. PMID: 26756094
A comprehensive updated review of already reported and newly identified SMPD1 variants of Niemann-Pick Types A and B disease has been presented. (Review) PMID: 26499107
a conserved haplotype and shared 280 Kb region around the SMPD1 gene was observed in the patients analyzed, indicating that the variant originated from a common ancestor PMID: 25920558
ASM activation may be involved in the pathophysiology of Kawasaki disease PMID: 26447086
These results indicate that increased EGR1/3 and ASMase expression play an important role in cellular ceramide increase by RSV treatment. PMID: 26809095
The results of this study suggested that disruptive mutations in SMPD1 constitute a risk factor for parkindon disease. PMID: 26169695
This is the first evidence that supports the possibility that sphingolipid metabolism is affected via the induction of ASMase by the Nrf2 pathway. PMID: 25762726
ASM has a pivotal role in adaptive immune T-cell responses. PMID: 26203857
Patterns of alternatively spliced SMPD1 transcripts are significantly different in patients with systemic inflammatory response syndrome and severe sepsis/septic shock compared to control subjects allowing discrimination of respective disease entity. PMID: 25898364
The mechanisms by which pyocyanin induces the release of mitochondrial ROS and by which ROS induce neutrophil death via mitochondrial acid sphingomyelinase was identified. PMID: 25686490
Results show four novel mutations in SMPD1 in Iranian patients with type A or B Niemann-Pick disease extending the genotypic spectrum of the disease. PMID: 25811928
The relationship between autophagy and A-SMase. [Review] PMID: 25666706
Data suggest ASM (acid sphingomyelinase) activity is regulated by membrane lipids and facilitates cholesterol transfer by NPC2 (Niemann Pick protein type C2); hydrolysis of sphingomyelin by ASM may be crucial for endosomal lipid degradation/sorting. PMID: 25339683
Data suggest invasiveness of Neisseria meningitidis depends on activation of SMPD1 and up-regulation of ceramide release to form ceramide-enriched platforms in cell membrane of endothelium of brain microvessels upon attachment of N. meningitidis. PMID: 24945304
L-ASM activity was significantly lower in subjects homozygous for the minor A allele but not different between allergic and non-allergic subjects PMID: 24977483
results provide the mechanism for dysferlin-mediated repair of skeletal muscle sarcolemma and identify ASM as a potential therapy for dysferlinopathy PMID: 24967968
These results reveal a novel mechanism of ASM pathogenesis in Alzheimer's disease. PMID: 25049335
The lysosomal enzyme coding genes SMPD1 which are associated with a 9-fold increased risk of Parkinson disease. PMID: 24262184
seven novel SMPD1 mutations and new inside into genotype/phenotype correlations in Niemann-Pick disease PMID: 23252888
Serum acid sphingomyelinase is upregulated in chronic hepatitis C infection and non-alcoholic fatty liver disease. PMID: 24769340
liver samples from patients with alcoholic hepatitis exhibited increased expression of ASMase, StARD1, and ER stress markers PMID: 23707365
Rare SMPD1 variant (p.R591C) increases the risk of Parkinson disease. PMID: 23871123
Mutational analysis demonstrated the siblings are compound heterozygotes (V112M and H554Y). PMID: 22367733
the spectrum of SMPD1 gene mutations in Turkish Niemann-Pick disease patients PMID: 23618813
Role of the acid sphingomyelinase (Asm)-ceramide system as a target for antidepressants. PMID: 23770692
Chinese population may have a comparably high incidence of sphingomyelinase-deficient Niemann-Pick disease type A. This study has identified some novel genotype and phenotype correlations in this rare and devastating disorder PMID: 23356216
The SMPD1 p.L302P mutation is a novel risk factor for Parkinson disease PMID: 23535491
Hydrogen peroxide, a primary form of reactive oxygen species in mammalian cells, induces very rapid translocation of ASM and formation of ceramide-enriched membrane platforms in the plasma membrane of Jurkat T cells. PMID: 22890197

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Involvement in disease

Niemann-Pick disease A (NPDA); Niemann-Pick disease B (NPDB)

Subcellular Location

Lysosome. Lipid droplet. Secreted.

Protein Families

Acid sphingomyelinase family

Database Links

HGNC: 11120

OMIM: 257200

KEGG: hsa:6609

STRING: 9606.ENSP00000340409

UniGene: Hs.498173

Code	CSB-EP021845HUa2
Abbreviation	Recombinant Human SMPD1 protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Sphingomyelin phosphodiesterase (SMPD1)

Product Details

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