Recombinant Human Spindle and kinetochore-associated protein 1 (SKA1)

Code CSB-YP846589HU
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Source Yeast
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Code CSB-EP846589HU
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Source E.coli
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Code CSB-EP846589HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846589HU
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Source Baculovirus
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Code CSB-MP846589HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SKA1
Uniprot No.
Alternative Names
2810433K01Rik; AV117428; C18orf24; MGC10200; Ska1; SKA1_HUMAN; Spindle and kinetochore associated complex subunit 1; Spindle and kinetochore associated protein 1; Spindle and kinetochore-associated protein 1; Spindle and KT (kinetochore) associated 1; Spindle and KT associated 1
Species
Homo sapiens (Human)
Expression Region
2-255
Target Protein Sequence
ASSDLEQLC SHVNEKIGNI KKTLSLRNCG QEPTLKTVLN KIGDEIIVIN ELLNKLELEI QYQEQTNNSL KELCESLEED YKDIEHLKEN VPSHLPQVTV TQSCVKGSDL DPEEPIKVEE PEPVKKPPKE QRSIKEMPFI TCDEFNGVPS YMKSRLTYNQ INDVIKEINK AVISKYKILH QPKKSMNSVT RNLYHRFIDE ETKDTKGRYF IVEADIKEFT TLKADKKFHV LLNILRHCRR LSEVRGGGLT RYVIT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.
Gene References into Functions
  1. decreased neuronal cell SKA complex genes' expression levels affect neuronal cell viability and neurite development PMID: 29268205
  2. High SKA1 expression is associated with early recurrence and progression in patients with NMIBC, indicating SKA1 may serve as a promising prognostic biomarker for this disease. PMID: 29865039
  3. End-binding protein 1 (EB1) depletion results in a significant reduction of spindle and kinetochore-associated protein 1 (Ska1) recruitment onto microtubules and defects in mitotic chromosome alignment. PMID: 27225956
  4. SKA1 might be a promising target for cancer gene therapy in human ACC. PMID: 28340379
  5. Results show that SKA1 expression is regulated by mir-10a. Its knockdown inhibits migration and invasion in renal cell carcinoma cells. PMID: 28746769
  6. Study indicated that hypoxia mediated downregulation of SKA1 expression increased the chemotherapy resistance in human osteosarcoma cells. PMID: 28278080
  7. Kinetochores mature through Ska1/ska2/Ska3 complex recruitment and this is required for improved load-bearing capacity and silencing of the spindle assembly checkpoint. PMID: 28495837
  8. In conclusion, our findings suggest that spindle and kinetochore-associated protein 1 could serve as a potential therapeutic target in prostate cancer patients. PMID: 28651496
  9. expression positively associated with tumor-node-metastasis stage and recurrence of salivary adenoid cystic carcinoma PMID: 26881872
  10. Thus, the Ska complex, specifically the Ska1 C-terminal domain, recruits PP1 to kinetochores to oppose spindle checkpoint signaling kinases and promote anaphase onset. PMID: 26981768
  11. SKA1 is required for metastasis and cisplatin resistance of non-small cell lung cancer. PMID: 26985856
  12. knockdown of SKA1 could potently suppress bladder cancer cell proliferation in vitro and lentivirus-mediated silencing of SKA1 might serve as a novel strategy for gene therapy of bladder cancer. PMID: 26197237
  13. Results show that high SKA1 expression is predictive of poor prognosis of papillary thyroid carcinoma (PTC), implying it as a promising new target for PTC therapies. PMID: 26063960
  14. SKA1 could be used for gastric cancer early diagnosis as a biomarker. PMID: 24627241
  15. the kinetochore-associated Ska complex interacts with tubulin monomers via the carboxy-terminal winged-helix domain of Ska1, providing the structural basis for the ability to bind both straight and curved microtubule structures. PMID: 24413531
  16. Ska1 over-expression promotes tumourigenesis PMID: 24827423
  17. Data suggest that SKA1 plays an important role in the proliferation of hepatocellular carcinoma cells PMID: 24010405
  18. the conserved kinetochore-localized Ska1 complex tracks with depolymerizing microtubule ends and associates with both the microtubule lattice and curved protofilaments PMID: 23085020
  19. The structure of the Ska core complex is a W-shaped dimer of coiled coils, formed by intertwined interactions between Ska1, Ska2, and Ska3. PMID: 22483620
  20. Data suggest that Aurora B phosphorylation antagonizes the interaction between the Ska1-3 complex and the KMN network, thereby controlling Ska recruitment to KTs and stabilization of KT-MT attachments. PMID: 22371557
  21. These data suggest that the Ska1/Ska2 complex plays a critical role in the maintenance of the metaphase plate and/or spindle checkpoint silencing. PMID: 17093495
  22. The full Ska1 complex forms assemblies on microtubules that can facilitate the processive movement of microspheres along depolymerizing microtubules. PMID: 19289083

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Subcellular Location
Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.
Protein Families
SKA1 family
Database Links

HGNC: 28109

OMIM: 616673

KEGG: hsa:220134

STRING: 9606.ENSP00000285116

UniGene: Hs.134726

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