Recombinant Human Structural maintenance of chromosomes protein 3 (SMC3), partial

Code CSB-YP891469HU
MSDS
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Source Yeast
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Code CSB-EP891469HU
MSDS
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Source E.coli
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Code CSB-EP891469HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891469HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP891469HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
BAM; Bamacan; Basement membrane associated chondroitin proteoglycan; Basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; chondroitin sulfate proteoglycan 6 (bamacan); Chondroitin sulfate proteoglycan 6; Chromosome associated polypeptide; Chromosome-associated polypeptide; CSPG 6; CSPG6; hCAP; Human chromosome associated polypeptide; im:7142991; SMC 3; SMC protein 3; SMC-3; smc3; SMC3_HUMAN; SMC3L1; Structural maintenance of chromosome 3; Structural maintenance of chromosomes 3; Structural maintenance of chromosomes protein 3; u:fb22e01; wu:fc30d07
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
Gene References into Functions
  1. H2 inhibited lung cancer progression through down-regulating SMC3. PMID: 29852353
  2. We identified a large number of mutations in the CC region of both Smc1 and Smc3... we introduced them to the yeast Smc1 and Smc3 CC domains and characterized the effect of these mutant alleles on cohesin's function. We identified a missense mutation in the region of the kink domain of Smc3, which was previously identified in kidney carcinoma PMID: 27307603
  3. Results show that SMC3 is upregulated in asthma patients and suggest that it may play an important role in atopic asthma development, especially in asthma epithelial-mesenchymal transition. PMID: 25515564
  4. Data show that histone deacetylase 8 (HDAC8) inhibition led to accumulation of acetylated-SMC3 protein but had no influence on the transcription of estrogen-responsive genes. PMID: 27072133
  5. De novo heterozygous mutations in SMC3 cause a range of Cornelia de Lange syndrome-overlapping phenotypes PMID: 25655089
  6. cohesin's proposed DNA exit gate is formed by interactions between Scc1 and the coiled-coil region of Smc3. PMID: 25414306
  7. Cross-sectional deep-sequencing analysis for clonal hierarchy demonstrated STAG2, SMC3, and RAD21 mutations to be ancestral in 18%, 18%, and 47% of cases, respectively, and each expanded to clonal dominance concordant with disease transformation PMID: 25006131
  8. Mutations in SMC3 is associated with acute myeloid leukemia. PMID: 24335498
  9. Upon knock-down of human SMC1, much of SMC3 remains stable, accumulates in the cytoplasm and does not associate with other cohesin proteins. PMID: 23776448
  10. SMC3 and separase are upregulated and securin is downregulated in malignant transformation of BEAS-2B cells induced by coal tar pitch smoke extracts. PMID: 21126432
  11. these studies clearly suggest that bamacan interacts with the vaccinia virus-N1L and such interactions seem to play a positive role in promoting the viral growth and perhaps contribute to the virulence of VV in neural cells. PMID: 19444697
  12. identification as target for beta-catenin/T-cell factor 4 transactivation pathway PMID: 12651860
  13. Hinderin is a novel binding partner of SMC3. Based on its ability to modulate SMC1/SMC3 interaction we postulate that Hinderin affects the availability of SMC3 to engage in the formation of multimeric protein complexes PMID: 15656913
  14. RPGR-ORF15, which is mutated in retinitis pigmentosa, associates with SMC3. PMID: 16043481
  15. RhoB and cAMP cis-acting response elements -BPa two known oncogenic mediators whose expression is significantly increased following SMC3 overexpression play a significant role in mediating SMC3 tumorigenesis PMID: 16156898
  16. SMC3 deficiency affects chromosomal stability leading to the activation of p53-dependent mitotic checkpoint. PMID: 17081288
  17. Mutations in SMC3 cause a mild variant of cornelia de Lange syndrome with predominant mental retardation PMID: 17273969
  18. phosphorylation of a core cohesin subunit SMC3 by ATM plays an important role in DNA damage PMID: 18442975
  19. This study identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion. PMID: 18614053
  20. There were greater levels of chondroitin sulphate WF6 epitope in gingival crevicular fluid from destructive sites of patients with chronic periodontitis compared with non-destructive sites of patients with gingivitis or chronic periodontitis. PMID: 18727658
  21. Data describe alterations of myocardial intercellular and cell-matrix contacts in hypertrophic tissue, and show intracellular translocation of beta-catenin, alpha-actinin and chondroitin sulfate proteoglycan 6 in both an animal model and in LVH patients. PMID: 19094982

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Involvement in disease
Cornelia de Lange syndrome 3 (CDLS3)
Subcellular Location
Nucleus. Chromosome. Chromosome, centromere.
Protein Families
SMC family, SMC3 subfamily
Database Links

HGNC: 2468

OMIM: 606062

KEGG: hsa:9126

STRING: 9606.ENSP00000354720

UniGene: Hs.24485

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