SMC3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA966408A0HU
Size	US$210
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Image	Western Blot Positive WB detected in:A431 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), SW620 whole cell lysate(30µg), Hela whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg), Rat brain tissue lysate(30µg) All lanes: SMC3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 142 kDa Observed band size: 142 kDa Exposure time：40s IHC image of CSB-RA966408A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA966408A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A431 cell with CSB-RA966408A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Raji cells stained with CSB-RA966408A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9UQE7

Target Names

SMC3

Alternative Names

Structural maintenance of chromosomes protein 3 (SMC protein 3) (SMC-3) (Basement membrane-associated chondroitin proteoglycan) (Bamacan) (Chondroitin sulfate proteoglycan 6) (Chromosome-associated polypeptide) (hCAP), SMC3, BAM BMH CSPG6 SMC3L1

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human SMC3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

SMC3 serves as a core component of the cohesin complex, playing an essential role in sister chromatid cohesion during cell division, DNA repair, and chromatin organization. This structural maintenance of chromosomes protein is fundamental to understanding chromosome dynamics and has significant implications for research into cell cycle regulation, genome stability, and cancer biology where cohesin dysfunction has been implicated.

This recombinant monoclonal antibody, developed using rabbit host immunization against a synthetic peptide derived from human SMC3, offers the consistency and reliability that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can compromise longitudinal studies and ensures reproducible results across your research timeline. The monoclonal nature of clone 12B7 guarantees specificity to a single epitope, providing clean, interpretable data.

Extensive validation demonstrates this antibody's versatility across multiple experimental platforms. Western blot analysis confirms detection of SMC3 at the expected 142 kDa molecular weight across diverse sample types including human cell lines such as A431, Jurkat, SW620, and HeLa, as well as mouse NIH/3T3 cells and rat brain tissue, confirming robust cross-species reactivity. Immunohistochemistry applications have been validated in human glioma and testis tissue sections, while immunofluorescence studies in A431 cells reveal clear nuclear localization patterns consistent with SMC3's chromatin-associated function. Flow cytometry validation in Raji cells further expands your analytical options.

Whether investigating chromosome segregation mechanisms, exploring cohesin complex dynamics in cancer models, or studying DNA damage response pathways, this antibody provides a reliable tool for advancing your research objectives.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.

Gene References into Functions

H2 inhibited lung cancer progression through down-regulating SMC3. PMID: 29852353
We identified a large number of mutations in the CC region of both Smc1 and Smc3... we introduced them to the yeast Smc1 and Smc3 CC domains and characterized the effect of these mutant alleles on cohesin's function. We identified a missense mutation in the region of the kink domain of Smc3, which was previously identified in kidney carcinoma PMID: 27307603
Results show that SMC3 is upregulated in asthma patients and suggest that it may play an important role in atopic asthma development, especially in asthma epithelial-mesenchymal transition. PMID: 25515564
Data show that histone deacetylase 8 (HDAC8) inhibition led to accumulation of acetylated-SMC3 protein but had no influence on the transcription of estrogen-responsive genes. PMID: 27072133
De novo heterozygous mutations in SMC3 cause a range of Cornelia de Lange syndrome-overlapping phenotypes PMID: 25655089
cohesin's proposed DNA exit gate is formed by interactions between Scc1 and the coiled-coil region of Smc3. PMID: 25414306
Cross-sectional deep-sequencing analysis for clonal hierarchy demonstrated STAG2, SMC3, and RAD21 mutations to be ancestral in 18%, 18%, and 47% of cases, respectively, and each expanded to clonal dominance concordant with disease transformation PMID: 25006131
Mutations in SMC3 is associated with acute myeloid leukemia. PMID: 24335498
Upon knock-down of human SMC1, much of SMC3 remains stable, accumulates in the cytoplasm and does not associate with other cohesin proteins. PMID: 23776448
SMC3 and separase are upregulated and securin is downregulated in malignant transformation of BEAS-2B cells induced by coal tar pitch smoke extracts. PMID: 21126432
these studies clearly suggest that bamacan interacts with the vaccinia virus-N1L and such interactions seem to play a positive role in promoting the viral growth and perhaps contribute to the virulence of VV in neural cells. PMID: 19444697
identification as target for beta-catenin/T-cell factor 4 transactivation pathway PMID: 12651860
Hinderin is a novel binding partner of SMC3. Based on its ability to modulate SMC1/SMC3 interaction we postulate that Hinderin affects the availability of SMC3 to engage in the formation of multimeric protein complexes PMID: 15656913
RPGR-ORF15, which is mutated in retinitis pigmentosa, associates with SMC3. PMID: 16043481
RhoB and cAMP cis-acting response elements -BPa two known oncogenic mediators whose expression is significantly increased following SMC3 overexpression play a significant role in mediating SMC3 tumorigenesis PMID: 16156898
SMC3 deficiency affects chromosomal stability leading to the activation of p53-dependent mitotic checkpoint. PMID: 17081288
Mutations in SMC3 cause a mild variant of cornelia de Lange syndrome with predominant mental retardation PMID: 17273969
phosphorylation of a core cohesin subunit SMC3 by ATM plays an important role in DNA damage PMID: 18442975
This study identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion. PMID: 18614053
There were greater levels of chondroitin sulphate WF6 epitope in gingival crevicular fluid from destructive sites of patients with chronic periodontitis compared with non-destructive sites of patients with gingivitis or chronic periodontitis. PMID: 18727658
Data describe alterations of myocardial intercellular and cell-matrix contacts in hypertrophic tissue, and show intracellular translocation of beta-catenin, alpha-actinin and chondroitin sulfate proteoglycan 6 in both an animal model and in LVH patients. PMID: 19094982

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Involvement in disease

Cornelia de Lange syndrome 3 (CDLS3)

Subcellular Location

Nucleus. Chromosome. Chromosome, centromere.

Protein Families

SMC family, SMC3 subfamily

Database Links

HGNC: 2468

OMIM: 606062

KEGG: hsa:9126

STRING: 9606.ENSP00000354720

UniGene: Hs.24485

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cell cycle

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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