Recombinant Human T-box transcription factor TBX20 (TBX20)

Code CSB-YP892476HU
MSDS
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Source Yeast
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Code CSB-EP892476HU
MSDS
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Source E.coli
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Code CSB-EP892476HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892476HU
MSDS
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Source Baculovirus
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Code CSB-MP892476HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TBX20
Uniprot No.
Alternative Names
T-box 20; T-box protein 20; T-box transcription factor TBX20; Tbox 20; tbx20; TBX20_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-447
Target Protein Sequence
MEFTASPKPQ LSSRANAFSI AALMSSGGSK EKEATENTIK PLEQFVEKSS CAQPLGELTS LDAHGEFGGG SGSSPSSSSL CTEPLIPTTP IIPSEEMAKI ACSLETKELW DKFHELGTEM IITKSGRRMF PTIRVSFSGV DPEAKYIVLM DIVPVDNKRY RYAYHRSSWL VAGKADPPLP ARLYVHPDSP FTGEQLLKQM VSFEKVKLTN NELDQHGHII LNSMHKYQPR VHIIKKKDHT ASLLNLKSEE FRTFIFPETV FTAVTAYQNQ LITKLKIDSN PFAKGFRDSS RLTDIERESV ESLIQKHSYA RSPIRTYGGE EDVLGDESQT TPNRGSAFTT SDNLSLSSWV SSSSSFPGFQ HPQSLTALGT STASIATPIP HPIQGSLPPY SRLGMPLTPS AIASSMQGSG PTFPSFHMPR YHHYFQQGPY AAIQGLRHSS AVMTPFV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a transcriptional activator and repressor required for cardiac development and may have key roles in the maintenance of functional and structural phenotypes in adult heart.
Gene References into Functions
  1. TBX20 can be considered a KCNH2-modifying gene. PMID: 28049825
  2. This study firstly links TBX20 loss-of-function mutation to familial tetralogy of Fallot or sporadic persistent truncus arteriosus, providing novel insight into the molecular pathogenesis of Congenital heart disease. PMID: 28553164
  3. results showed that the TBX20 gene is not the major gene affecting nonsyndromic congenital heart disease development PMID: 28525297
  4. chromatin analysis reveals that endocardial TBX20 has roles in septation PMID: 27348591
  5. The current study associated TBX20 haploinefficiency with isolated Dilated cardiomyopathy (DCM), and expanded upon the mutational spectrum of TBX20 associated with DCM and congenital heart disease (CHD), which provides novel insight into the molecular mechanism of DCM and CHD, suggesting potential implications for early personalized treatment of these diseases. PMID: 27510170
  6. Silencing of TBX20 in rat myocardial and human embryonic kidney cells significantly inhibited cell proliferation, induced cell apoptosis and led to G2/M cell cycle arrest. PMID: 27572266
  7. rs3999950 may be associated with congenital heart disease, and TBX20 may predispose children to the defect. PMID: 27323105
  8. Among the 8 SNPs identified, 6 are in strong linkage disequilibrium and the minor alleles are associated with lower CHD risk. The minor alleles have lower transcriptional activity than major alleles in both human heart tissues and three cell lines. TBX20 minor alleles may exhibit higher binding affinity with certain transcription repressors. PMID: 27034249
  9. Cardiac TBX20 expression showed a negative correlation with LVEF and a positive correlation with left ventricular end-systolic volume. No significant difference in TBX20 CNVs and promoter methylation was observed between IDCM patients and control group PMID: 26895318
  10. The mutation markedly reduced the synergistic activation of TBX20 with NKX2-5 or GATA4. PMID: 26118961
  11. We report three missense mutations (Y309D, T370O, and M395R) within the transcriptional activator domain of human TBX20 that were associated with atrial septal defect PMID: 25834824
  12. Data showed that the TC genotype of SNP rs3999941 and AC genotype of the new SNP c.657A>C in the TBX20 gene may be risk factors for CHD. PMID: 25487630
  13. TBX20 loss-of-function mutation contributes to double outlet right ventricle PMID: 25625280
  14. A novel TBX20 mutation, c.526G>A (p.D176N), was identified and co-segregated in all affected members in this family of three generations with atrial septal defects. PMID: 25183037
  15. Tbx20 regulated PPAR-gamma expression and protected the vascular endothelial cells from oxidized low-density lipoprotein -induced injury. PMID: 24247152
  16. Tbx20 functions as an important regulator of estrogen-mediated cardiomyocyte protection during oxidative stress. PMID: 23871353
  17. Study identified one novel heterozygous sequence variant within the proximal promoter region of TBX20 gene in a ventricular septal defects patient, which inhibited transcriptional activities of TBX20 gene promoter; data provide new information to help understanding of genetic causes and molecular mechanisms of congenital heart disease. PMID: 22465533
  18. This novel interaction between TBX20b and MKLN1 may help elucidate new regulatory mechanisms within heart development. PMID: 21586270
  19. Found tertiary hydrophobic interactions within the mutant TBX20 T-box of Ostium secundum atrial septal defect subjects were significantly altered leading to a more dynamic structure of the protein. PMID: 19762328
  20. Our findings are the first to link TBX20 mutations to human pathology PMID: 17668378
  21. analysis of TBX20 in human hearts and its regulation by TFAP2 PMID: 18275040
  22. findings provide the first insight into TBX20 mutations for tetrology of fallot and anomalous pulmonary venous connection PMID: 18834961
  23. These data highlight unique features of Tbx20 and suggest mechanistic ways in which cardiac T-box factors might interact synergistically and/or competitively within the cardiac regulatory network. PMID: 19414016

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Involvement in disease
Atrial septal defect 4 (ASD4)
Subcellular Location
Nucleus.
Database Links

HGNC: 11598

OMIM: 606061

KEGG: hsa:57057

STRING: 9606.ENSP00000386170

UniGene: Hs.404167

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