Recombinant Human T-lymphoma invasion and metastasis-inducing protein 2 (TIAM2), partial

Code CSB-YP818235HU
MSDS
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Source Yeast
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Code CSB-EP818235HU
MSDS
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Source E.coli
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Code CSB-EP818235HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818235HU
MSDS
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Source Baculovirus
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Code CSB-MP818235HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIAM2
Uniprot No.
Alternative Names
FLJ41865; OTTHUMP00000017472; SIF and TIAM1 like exchange factor ; SIF and TIAM1-like exchange factor; STEF ; T cell lymphoma invasion and metastasis 2; T lymphoma invasion and metastasis inducing protein 2; T-lymphoma invasion and metastasis-inducing protein 2; TIAM 2; TIAM-2; Tiam2; TIAM2_HUMAN
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane. Activates specifically RAC1, but not CDC42 and RHOA.
Gene References into Functions
  1. The authors show that the Rac1 selective guanine nucleotide exchange factor Sif and TIAM1-like Exchange Factor STEF, also known as TIAM2, localises to the nuclear envelope, co-localises with key perinuclear proteins and regulates the activity of perinuclear Rac1. PMID: 29844364
  2. Tiam2/Rac are key components of EphB2 trans-endocytosis and are important for cell repulsion. PMID: 27597758
  3. Results provide evidence that Sp1 positively controls TIAM2S transcription and that Sp1-mediated transcriptional activation is essential for TIAM2S ectopic expression in liver cancer cells. PMID: 26763486
  4. Knockdown of TIAM2 could up-regulate the expression of E-cadherin. PMID: 24377522
  5. TIAM2 expression was undetectable in normal human liver but was induced in all HCC cell lines and in 86% (78/91) of HCC biopsies. PMID: 21469146
  6. stef gene expressed in a stage- and region-specific manner in the mouse brain PMID: 11900975
  7. STEF protein is a guanine nucleotide exchange factor that specifically activates Rac1 in vitro and in cultured cells, which may lead to changes in the actin cytoskeletal network. PMID: 10364228

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Subcellular Location
Cytoplasm. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, growth cone. Cell projection, neuron projection. Perikaryon.
Protein Families
TIAM family
Tissue Specificity
Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis.
Database Links

HGNC: 11806

OMIM: 604709

KEGG: hsa:26230

STRING: 9606.ENSP00000327315

UniGene: Hs.586279

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