Recombinant Human TATA-binding protein-associated factor 172 (BTAF1), partial

Code CSB-YP002839HU
MSDS
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Source Yeast
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Code CSB-EP002839HU
MSDS
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Source E.coli
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Code CSB-EP002839HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002839HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP002839HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BTAF1
Uniprot No.
Alternative Names
ATP dependent helicase BTAF1; ATP-dependent helicase BTAF1; B-TFIID transcription factor associated 170 kDa subunit ; B-TFIID transcription factor associated, 170kDa (Mot1 homolog, S. cerevisiae); B-TFIID transcription factor-associated 170 kDa subunit; BTAF 1 RNA polymerase II; BTAF1; BTAF1 RNA polymerase II B TFIID transcription factor associated 170kDa; BTAF1_HUMAN; MOT 1; MOT1; MOT1 Yeast homolog of; TAF 172; TAF II 170; TAF(II)170; TAF-172; TAF172; TAFII170; TATA binding protein associated factor 172; TATA-binding protein-associated factor 172; TBP associated factor 172; TBP-associated factor 172
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
Gene References into Functions
  1. Results support a model for a BTAF1-mediated release of TBP-NC2 complexes from chromatin. PMID: 20627952
  2. study with the first structural model of the B-TFIID complex and map of its key functional domains PMID: 14988402
  3. physical cooperation between BTAF1 and NC2alpha in TBP regulation PMID: 15509807
  4. We propose that these density rearrangements in the structure are a likely reflection of the plasticity of the interactions between TFIID and its many partner proteins. PMID: 16531235
  5. histone acetylation, although it occurs, is dispensable for TFIID and SWI/SNF recruitment by the strong enhancers PMID: 18025106

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Subcellular Location
Nucleus.
Protein Families
SNF2/RAD54 helicase family
Database Links

HGNC: 17307

OMIM: 605191

KEGG: hsa:9044

STRING: 9606.ENSP00000265990

UniGene: Hs.500526

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