Recombinant Human TBC domain-containing protein kinase-like protein (TBCK), partial

Code CSB-YP840579HU
MSDS
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Source Yeast
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Code CSB-EP840579HU
MSDS
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Source E.coli
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Code CSB-EP840579HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840579HU
MSDS
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Source Baculovirus
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Code CSB-MP840579HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TBCK
Uniprot No.
Alternative Names
Hypothetical protein MGC16169; IHPRF3; MGC16169; TBC domain containing protein kinase like; TBC domain containing protein kinase like protein; TBC domain-containing protein kinase-like protein; TBC1 domain containing kinase; TBCK; TBCK_HUMAN; TBCKL
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the modulation of mTOR signaling and expression of mTOR complex components. Involved in the regulation of cell proliferation and growth. Involved in the control of actin-cytoskeleton organization.
Gene References into Functions
  1. We conclude that the c.1854delT variant in the TBCK gene is the mutation causing the congenital brain abnormality in an Arab-Moslem family from northern Israel. PMID: 27748029
  2. RNAsequencing showed that the t(4;5)(q24;q31) resulted in recombination of the genes TBCK on 4q24 and P4HA2 on 5q31.1 with generation of an inframe TBCKP4HA2 and the reciprocal but outofframe P4HA2TBCK fusion transcripts. PMID: 27633981
  3. We have reported a series of 13 individuals from nine unrelated families that harbor biallelic mutation in TBCK and display overlapping features of intellectual disability and hypotonia. This condition is called TBCK-related intellectual disability syndrome. PMID: 27040691
  4. We have established that biallelic mutations in TBCK cause a severe neurodevelopmental disorder whose major features include profound developmental delay or cognitive deficit, brain atrophy without microcephaly. PMID: 27040692
  5. localization and function of TBCK PMID: 24576458
  6. TBCK may play an important role in cell proliferation, cell growth and actin organization possibly by modulating mTOR pathway. PMID: 23977024

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Involvement in disease
Hypotonia, infantile, with psychomotor retardation and characteristic facies 3 (IHPRF3)
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, spindle. Midbody.
Protein Families
Protein kinase superfamily
Database Links

HGNC: 28261

OMIM: 616899

KEGG: hsa:93627

STRING: 9606.ENSP00000273980

UniGene: Hs.151498

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