Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TAS1R1

Uniprot No.

Q7RTX1

Research Area

Neuroscience

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

21-567aa

Target Protein Sequence

FACHSTESSPDFTLPGDYLLAGLFPLHSGCLQVRHRPEVTLCDRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

68.1 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 21-567 constitute the expression domain of recombinant Human TAS1R1. The theoretical molecular weight of the TAS1R1 protein is 68.1 kDa. This protein is generated in a e.coli-based system. The TAS1R1 coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant TAS1R1 protein in following stages of expression and purification.

The human taste receptor type 1 member 1 (TAS1R1) is a G protein-coupled receptor (GPCR) that plays a key role in the perception of umami taste, which is associated with savory or meaty flavors. TAS1R1, along with TAS1R2, forms a heterodimeric receptor complex known as T1R1/T1R2, responsible for recognizing amino acids such as glutamate. Activation of TAS1R1/TAS1R2 by umami compounds triggers intracellular signaling cascades, leading to the perception of umami taste. This receptor is primarily expressed in taste receptor cells on the tongue, contributing to the complex sensory experience of taste by detecting specific chemical compounds in food. Understanding TAS1R1's function is crucial for unraveling the molecular basis of taste perception.

Target Background

Function

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.

Gene References into Functions

Genetic variants in TAS1R were associated with dietary consumption, which may be associated with gastric cancer risk PMID: 27321875
The study provides human psychophysical evidence that TAS1R1 polymorphisms associate with modest differences in overall taste intensity, which were independent from other well-studied markers of variation in oral sensation. PMID: 24000232
A complex molecular mechanism involving changes in the properties of both the orthosteric and non-orthosteric sites of T1R1 underlies the determination of ligand specificity in mammalian T1R1/T1R3. PMID: 24214976
Data show that that Tas1r1 and Tas1r3 are expressed in murine and human spermatozoa. PMID: 22427794
amino acid substitutions (A110V and R507Q) in the N-terminal ligand-binding domain of T1R1 severely impair receptor functions in vitro PMID: 21422378
sequence differences in T1R receptors within and between species (human and mouse) can significantly influence the selectivity and specificity of taste responses PMID: 11894099
findings demonstrate the different functional roles of T1R3 and T1R2 and the presence of multiple ligand binding sites on the sweet taste receptor PMID: 15353592
In vivo taste bud signaling may proceed primarily through hT1R1 and hT1R2. PMID: 17506496
human duodenal L cells express sweet taste receptor T1r. PMID: 17724330
Taken together, these results suggest that Tas1R1-Tas1R3 receptor variants found in human fungiform papillae might contribute to inter-individual differences of sensitivity to L-glutamate. PMID: 19146926
In our sample of subjects, the frequencies of 2 nsSNPs, C329T in tas1r1 and C2269T in tas1r3, were significantly higher in [glutamate] nontasters than expected, whereas G1114A in tas1r1 was more frequent in tasters. PMID: 19571223

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 3 family, TAS1R subfamily

Database Links

HGNC: 14448

OMIM: 606225

KEGG: hsa:80835

STRING: 9606.ENSP00000331867

UniGene: Hs.124574

Code	CSB-EP766405HU
Abbreviation	Recombinant Human TAS1R1 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Taste receptor type 1 member 1 (TAS1R1), partial

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