Recombinant Human Thioredoxin-related transmembrane protein 1 (TMX1), partial

Code CSB-YP023950HU
MSDS
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Source Yeast
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Code CSB-EP023950HU
MSDS
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Source E.coli
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Code CSB-EP023950HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023950HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP023950HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TMX1
Uniprot No.
Alternative Names
PDIA11; Protein disulfide isomerase family A, member 11; Thioredoxin domain containing 1; Thioredoxin domain containing; Thioredoxin domain-containing protein 1; Thioredoxin-related transmembrane protein 1; TMX; TMX1; TMX1_HUMAN; Transmembrane Trx-related protein; TXNDC; TXNDC1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May participate in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange reactions.
Gene References into Functions
  1. TMX1 selectively intervenes in clearance of membrane-tethered Endoplasmic Reticulum-Associated Degradation substrates. TMX1 preferentially acts on membrane-tethered folding-defective polypeptides essentially ignoring the same misfolded ectodomains, when not associated to the Endoplasmic Reticulum membrane. PMID: 29932915
  2. TMX1 increases mitochondrial ATP production and apoptosis progression. PMID: 27502484
  3. Here we show that TMX1, one of the few transmembrane members of the family, forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins PMID: 26246604
  4. We conclude that TMX plays a major role in host defense under the type of inflammatory conditions associated with oxidative stress. PMID: 22924822
  5. the silencing of TMX in the prostatic cell line DU145 reduced the sensitivity of the cells to ricin intoxication further confirming a role for this enzyme in intracellular ricin activation. PMID: 22228764
  6. TMX1 is enriched on the mitochondria-associated membrane. Targeting TMX to the MAM requires palmitoylation of two membrane-proximal cytosolic cysteines. PMID: 22045338
  7. These results suggest a specific role for transmembrane thioredoxin-related protein TMX and its mechanism of action in redox-based ER quality control. PMID: 19741092

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Subcellular Location
Membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Tissue Specificity
Ubiquitous. Highly expressed in kidney, liver, placenta and lung.
Database Links

HGNC: 15487

OMIM: 610527

KEGG: hsa:81542

STRING: 9606.ENSP00000393316

UniGene: Hs.125221

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