Recombinant Human Transcription factor HES-4 (HES4)

Code CSB-YP884518HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP884518HU
MSDS
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Source E.coli
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Code CSB-EP884518HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884518HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP884518HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HES4
Uniprot No.
Alternative Names
HES4; BHLHB42; Transcription factor HES-4; hHES4; Class B basic helix-loop-helix protein 42; bHLHb42; Hairy and enhancer of split 4; bHLH factor Hes4
Species
Homo sapiens (Human)
Expression Region
1-221
Target Protein Sequence
MAADTPGKPS ASPMAGAPAS ASRTPDKPRS AAEHRKSSKP VMEKRRRARI NESLAQLKTL ILDALRKESS RHSKLEKADI LEMTVRHLRS LRRVQVTAAL SADPAVLGKY RAGFHECLAE VNRFLAGCEG VPADVRSRLL GHLAACLRQL GPSRRPASLS PAAPAEAPAP EVYAGRPLLP SLGGPFPLLA PPLLPGLTRA LPAAPRAGPQ GPGGPWRPWL R
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'.
Gene References into Functions
  1. Hes4 overexpression promotes a more aggressive tumor phenotype by preventing osteoblastic differentiation of osteosarcoma cells. Hes4 expression may allow for the stratification of patients into good or poor responders to chemotherapy at diagnosis. PMID: 27786411
  2. Overexpression and knockdown studies demonstrated that Hes4 promotes osteogenesis resulting in an increase in Runx2, osteocalcin, osteopontin, and bone sialoprotein expression in BMSC. PMID: 25579220
  3. Epigenetic dysregulation of HES4 could play a critical role in modifying Huntington's disease pathogenesis and severity. PMID: 25480889
Subcellular Location
Nucleus.
Database Links

HGNC: 24149

OMIM: 608060

KEGG: hsa:57801

UniGene: Hs.154029

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