Recombinant Human Transcription factor HIVEP3 (HIVEP3), partial

Code CSB-YP719398HU
MSDS
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Source Yeast
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Code CSB-EP719398HU
MSDS
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Source E.coli
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Code CSB-EP719398HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP719398HU
MSDS
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Source Baculovirus
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Code CSB-MP719398HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HIVEP3
Uniprot No.
Alternative Names
HIVEP3; KBP1; KIAA1555; KRC; ZAS3; Transcription factor HIVEP3; Human immunodeficiency virus type I enhancer-binding protein 3; Kappa-B and V(D)J recombination signal sequences-binding protein; Kappa-binding protein 1; KBP-1; Zinc finger protein ZAS3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role of transcription factor; binds to recognition signal sequences (Rss heptamer) for somatic recombination of immunoglobulin and T-cell receptor gene segments; Binds also to the kappa-B motif of gene such as S100A4, involved in cell progression and differentiation. Kappa-B motif is a gene regulatory element found in promoters and enhancers of genes involved in immunity, inflammation, and growth and that responds to viral antigens, mitogens, and cytokines. Involvement of HIVEP3 in cell growth is strengthened by the fact that its down-regulation promotes cell cycle progression with ultimate formation of multinucleated giant cells. Strongly inhibits TNF-alpha-induced NF-kappa-B activation; Interferes with nuclear factor NF-kappa-B by several mechanisms: as transcription factor, by competing for Kappa-B motif and by repressing transcription in the nucleus; through a non transcriptional process, by inhibiting nuclear translocation of RELA by association with TRAF2, an adapter molecule in the tumor necrosis factor signaling, which blocks the formation of IKK complex. Interaction with TRAF proteins inhibits both NF-Kappa-B-mediated and c-Jun N-terminal kinase/JNK-mediated responses that include apoptosis and proinflammatory cytokine gene expression. Positively regulates the expression of IL2 in T-cell. Essential regulator of adult bone formation.
Gene References into Functions
  1. rheumatoid arthritis-fibroblast-like synoviocytes were more sensitive to the synergistic increase of Schnurri-3 expression. PMID: 27072520
  2. Transdifferentiation of VSMC into calcifying vascular cells is enhanced by LPC and attenuated by Schnurri-3. PMID: 23500015
  3. ZAS3 expression is directly regulated by estrogen and is overexpressed in lupus. Its upregulation by estrogen could play a critical role in female-biased autoimmune disorders. PMID: 23178823
  4. The inhibitory effect of ZAS3 on NF-kappa B activity is mediated by neither direct association with N-kappa B nor disrupting nuclear localization of NFvarkappaB. PMID: 21524353
  5. DNA competition by ZAS3 is sufficient to repress transcription activated by NF-kappaB and that ZAS3 is an endogenous kappaB-motif competitor with ininsic repression activity. PMID: 21189157
  6. An interaction between ZAS3 and Smad proteins enhances transforming growth factor beta signaling. PMID: 20732416
  7. Overexpression of KRC in transformed and primary T cells leads to increased IL-2 production. PMID: 14707112
  8. Single nucleotide polymorphisms strongly support HIVEP3 as a candidate for PARK10 in Parkinson disease. PMID: 17388942

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Subcellular Location
Cytoplasm. Nucleus.
Database Links

HGNC: 13561

OMIM: 606649

KEGG: hsa:59269

STRING: 9606.ENSP00000247584

UniGene: Hs.403972

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