Recombinant Human Transcription initiation factor IIB(GTF2B)

Code CSB-EP010001HU
Size $1812Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names GTF2B
Uniprot No. Q00403
Research Area Immunology
Alternative Names General transcription factor IIB; General transcription factor TFIIB; gtf2b; RNA polymerase II transcription factor IIB; S300 II; S300-II; TF IIB; TF2B; TF2B_HUMAN; TFIIB; Transcription initiation factor IIB
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-316aa
Target Protein Sequence MASTSRLDALPRVTCPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLPKQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYRLIYPRAPDLFPTDFKFDTPVDKLPQL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 50.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.
Gene References into Functions
  1. a hand-off model in which Ssu72 mediates a conformational transition in TFIIB, accounting for the role of Ssu72 in transcription reinitiation, gene looping, and promoter-terminator cross-talk. PMID: 29158257
  2. binding of specific DNA sequences changes the protein structure and dynamics, and TFIIB may exist in two conformational states PMID: 26284261
  3. The role of zinc at Cys3His1 binding site in the absence of TFIIB protein folding PMID: 26016528
  4. these results establish a new paradigm for TFIIB functionality in human gene expression, which when downregulated has potent anti-viral effects. PMID: 24441171
  5. Association of the winged helix motif of the TFIIEalpha subunit of TFIIE with either the TFIIEbeta subunit or TFIIB distinguishes its functions in transcription. PMID: 25492609
  6. TFIIB was involved in the proliferation and growth of HCC cells. PMID: 23055019
  7. a mode of phospho-TFIIB-independent transcriptional regulation that prioritizes the transcription of p53-target genes during cellular stress. PMID: 23115335
  8. Data show that TFIIF has an important role in stabilizing TFIIB within the PIC and after transcription initiates. PMID: 21896726
  9. a six-amino acid human TFIIB tip region is needed for appropriate levels of serine 5 C-terminal domain phosphorylation and mRNA capping and for retention of the required elongation factor TFIIF. PMID: 20880846
  10. phosphorylation of TFIIB at serine 65 is a critical event in transcription that links the gene promoter and terminator and triggers initiation by RNA pol II PMID: 20226668
  11. interaction with vitamin D receptor helix H10 residues PMID: 12529369
  12. Data show that human TATA box binding protein (TBP) can use a shared surface to interact with two different transcription factor IIB (TFIIB) family members to initiate transcription by different RNA polymerases. PMID: 12535529
  13. The groove in the first cyclin repeat of TFIIB is identified as the FCP1-binding site of TFIIB core domain (TFIIBc), consisting of several hydrophobic and several basic amino acids. PMID: 12578358
  14. lack of role in bending of TATA-binding protein simultaneously with promotoer DNA PMID: 12791683
  15. TFIIB interacts with SP1/SP3 at the SP1 site, besides its association with EAR3 and the TATA-less core promoter region. PMID: 12972613
  16. in addition to its role in regulating TBP binding to a TATA box, the TBP surface is unexpectedly involved in TBP association with all three TFIIB family members PMID: 14585974
  17. The structural role of Zn2+ binding to the the zinc-ribbon domain of TFIIB was analysed. PMID: 14641108
  18. Results identify a small nonconserved surface of the transcription factor TFIIB zinc ribbon that is required for RNA polymerase II (pol II) transcription in vivo and for different types of basal pol II transcription in vitro. PMID: 15024075
  19. A TFIIB conformational change is critical for the formation of activator-dependent transcription complexes. PMID: 15037660
  20. Collapse of the transcription bubble defines the RNA polymerase II promoter clearance transition. PMID: 15989968
  21. high level of TFIIB can bypass the Mediator requirement for basal transcription and pol II recruitment in nuclear extract PMID: 16595664
  22. Together, our results support a mode of preinitiation complex assembly in which TFIIB/RNA polymerase II recruitment to the promoter occurs in vivo. PMID: 16878124
  23. TFIIB binding is required for the interferon antagonist effect exerted by Thogoto virus M protein. PMID: 18768974
  24. slipping at juncture is induced by TATA-binding protein and transcription factor IIB and requires a TATA box but not a transcription factor IIB recognition sequence PMID: 19193635
  25. TFIIF in HeLa cell nuclear extract helps to rescue the inactive mutations by interacting with either the B-finger or another component of the initiation complex that is influenced by the B-finger PMID: 19590095

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Subcellular Location Nucleus. Chromosome.
Protein Families TFIIB family
Tissue Specificity Expressed in the inner cell mass forming the embryoblast. Not detected in cells from the outer thin layer trophoblast (at protein level).
Database Links

HGNC: 4648

OMIM: 189963

KEGG: hsa:2959

STRING: 9606.ENSP00000359531

UniGene: Hs.481852

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