Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TKT

Uniprot No.

P29401

Research Area

Cardiovascular

Alternative Names

EC 2.2.1.1; FLJ34765; TK; TKT 1; tkt; TKT_HUMAN; TKT1; Transketolase (Wernicke Korsakoff syndrome); Transketolase

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-623aa

Target Protein Sequence

MESYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKSQDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLAEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCLLGDGELSEGSVWEAMAFASIYKLDNLVAILDINRLGQSDPAPLQHQMDIYQKRCEAFGWHAIIVDGHSVEELCKAFGQAKHQPTAIIAKTFKGRGITGVEDKESWHGKPLPKNMAEQIIQEIYSQIQSKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDRIIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRNRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPTSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYNNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAELLKKEKINIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEGGIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDAIAQAVRGLITKA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

67.9 kDa

Protein Length

Full Length

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

If I want to store for a long time and do not want to add glycerol, I can decide whether to choose the lyophilized protein according to the my experimental demand.
Does it mean that the I choose lyophilized protein or frozen protein?
Can you provide the concentration as lyophilization form?

Very nice to receive your inquiry. If you want to store for a long time and do not want to add glycerol,you can decide whether to choose the lyophilized protein according to the your experimental demand.
We mean if tyou doesn't want to add glycerol or only can accept a low concentration of glycerol, but you want to store the protein for a long time,
then it's better to choose the lyophilized form of the protein, because the protein without glycerol or low concentration of glycerol can't be stored for a long time, otherwise, it will be unstable.
You can choose a liquid protein with 5%-50% glycerol or a lyophilized protein without any glycerol. It decides by you.
The concentration of the lyophilized is dertermiend by you, as you can dissolve the protein and adjust the concentration by yourself.

If I would like to request a lyophilized protein without any glycerol, do I need to pay any additional cost?

Very nice to receive your inquiry. The lyophilization service is for free, that means you don't need to pay any cost for a lyophilized protein without any glycerol.

Target Background

Function

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.

Gene References into Functions

Over-expressed and hypo-methylated TKT gene is associated with hepatocellular carcinoma. PMID: 27760737
The observed associations of genetic variation in transketolase enzyme with neuropathic symptoms and reduced thermal sensation in recent-onset diabetes suggest a role of pathways metabolizing glycolytic intermediates in early diabetic neuropathy. PMID: 27103086
reduced expression of transketolase in pyrimidine 5'-nucleotidase deficient patients PMID: 27381654
Mutations in TKT gene is associated with a Syndrome Including Short Stature, Developmental Delay, and Congenital Heart Defects. PMID: 27259054
transketolase (TKT)is required for cancer growth because of its ability to affect the production of NAPDH to counteract oxidative stress. PMID: 26811478
Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation. PMID: 25895698
Akt1 phosphorylates TKT on Thr382, markedly enhancing enzyme activity. PMID: 24981175
TKT rs3736156 identified significant differences in the onset of cardiovascular event in patients having genotypes AG + AA versus GG PMID: 23492569
basal TK activity was decreased in cases with diabetic neuropathy PMID: 24114639
Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme. PMID: 22645655
Data indicate that transketolase (hTKT). shares 61% sequence identity with transketolase-like protein (TKTL1). PMID: 23261987
No transketolase activity of TKTDelta38 can be detected for conversion of physiological sugar substrates thus arguing against an intrinsically encoded enzymatic function of TKTL1 in tumor cell metabolism PMID: 23118983
TKT is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
Single Nucleotide Polymorphism in transketolase is associated with diabetic nephropathy. PMID: 20826743
The crystal structure of human transketolase and new insights into its mode of action. PMID: 20667822
Almost all multiple sclerosis patients had cerebrospinal fluid IgG directed to isoforms of one of the oligodendroglial molecules, transketolase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase type I, collapsin response mediator protein 2, and tubulin beta4. PMID: 18676363

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Involvement in disease

Short stature, developmental delay, and congenital heart defects (SDDHD)

Protein Families

Transketolase family

Database Links

HGNC: 11834

OMIM: 606781

KEGG: hsa:7086

STRING: 9606.ENSP00000405455

UniGene: Hs.89643

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