Recombinant Human Tripartite motif-containing protein 14 (TRIM14)

Code CSB-YP614516HU
MSDS
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Source Yeast
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Code CSB-EP614516HU
MSDS
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Source E.coli
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Code CSB-EP614516HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP614516HU
MSDS
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Source Baculovirus
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Code CSB-MP614516HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TRIM14
Uniprot No.
Alternative Names
5830400N10Rik ; KIAA0129; pub; TRI14_HUMAN; TRIM14; tripartite motif protein 14; tripartite motif protein TRIM14; tripartite motif-containing 14; Tripartite motif-containing protein 14
Species
Homo sapiens (Human)
Expression Region
1-442
Target Protein Sequence
MAGAATGSRT PGRSELVEGC GWRCPEHGDR VAELFCRRCR RCVCALCPVL GAHRGHPVGL ALEAAVHVQK LSQECLKQLA IKKQQHIDNI TQIEDATEKL KANAESSKTW LKGKFTELRL LLDEEEALAK KFIDKNTQLT LQVYREQADS CREQLDIMND LSNRVWSISQ EPDPVQRLQA YTATEQEMQQ QMSLGELCHP VPLSFEPVKS FFKGLVEAVE STLQTPLDIR LKESINCQLS DPSSTKPGTL LKTSPSPERS LLLKYARTPT LDPDTMHARL RLSADRLTVR CGLLGSLGPV PVLRFDALWQ VLARDCFATG RHYWEVDVQE AGAGWWVGAA YASLRRRGAS AAARLGCNRQ SWCLKRYDLE YWAFHDGQRS RLRPRDDLDR LGVFLDYEAG VLAFYDVTGG MSHLHTFRAT FQEPLYPALR LWEGAISIPR LP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an essential role in the innate immune defense against viruses and bacteria. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways. Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation. Acts as a scaffold between TBK1 and STAT3 to promote phosphorylation of STAT3 and resolve interferon-stimulated gene (ISG) expression. Inhibits the transcriptional activity of SPI1 in a dose-dependent manner.
Gene References into Functions
  1. Results showed that TRIM14 is increased in gastric cancer (GC) tissues and cell lines and associated with malignant features and unfavorable prognosis. Gain and lossoffunctional data confirmed that TRIM14 promotes migration, invasion and EMT progression by activating AKT signaling. TRIM14 was found to function as an oncogene in regulating EMT and metastasis of GC via AKT signaling, which was regulated by miR195. PMID: 30272351
  2. Our findings collectively suggest that TRIM14 functions as an oncogene by upregulating the AKT signaling pathway in osteosarcoma cells, supporting its potential utility as a therapeutic target for this disease. PMID: 28205534
  3. Study shows that tripartite Motif 14 (TRIM14) is a putative tumor suppressor and regulator of innate immune response in non-small cell lung cancer. The functional data establishes a novel tumor suppressive role for TRIM14 in non-small cell lung cancer progression. PMID: 28059079
  4. we identify the tripartite motif-containing protein (TRIM14) as a target of miR-195-5p. Therefore, we reason that the tumor suppressor role of miR-195-5p in oral squamous cell carcinoma is dependent on the interaction with TRIM14. PMID: 29204446
  5. In searching for mechanisms how TRIM14 exerts its antiviral function we found that TRIM14 interacted with HCV encoded non-structural protein NS5A and could strongly induce its degradation dependent on the NS5A1 subdomain. Interestingly extensive domain mapping analyses revealed that NS5A degradation was mediated by the highly conserved SPRY domain of TRIM14, which might involve the K48 ubiquitination pathway PMID: 27578425
  6. findings define the WHIP-TRIM14-PPP6C mitochondrial signalosome required for RIG-I-mediated innate antiviral immunity. PMID: 29053956
  7. study identifies new gene-type zinc finger protein 125 (RNF125) as a negative regulator of TRIM14 in the innate antiviral immune response PMID: 28476934
  8. survival of xenograft mice was prolonged by BsAbBmi/TRIM treatment compared to either AbBmi-1 or AbTRIM-14 treatment. In conclusion, these results provided new evidence that BsAbBmi/TRIM inhibited the progression of osteosarcoma, which suggest that BsAbBmi/TRIM may be a novel anti-cancer agent for osteosarcoma therapy PMID: 28631557
  9. MiR-15b degrades TRIM14 in oral tongue squamous cell cancer.TRIM14 role in oral tongue squamous cell cancer resistance to cisplatin. PMID: 28350138
  10. Data suggest that tripartite motif containing 14 protein (TRIM14) might play an important role in the malignant progression of tongue squamous cells carcinoma (TSCC) and in regulation of the NF_Kappa B (NF-kappaB) signaling pathway. PMID: 26799420
  11. stable enhanced expression of trim14 gene in cells activates the transcription of many immunity genes and suppresses Sindbis virus reproduction, but Sindbis virus infection of HEK-trim14 cells promotes inhibition of some genes involved in innate immunity. PMID: 25948474
  12. Upon virus infection, TRIM14 recruits NF-kappaB essential modulator (NEMO) to the MAVS complex via ubiquitin chains. PMID: 24379373

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Subcellular Location
Mitochondrion outer membrane. Cytoplasmic vesicle, phagosome.
Protein Families
TRIM/RBCC family
Tissue Specificity
Highest expression in liver; undetectable in skeletal muscle.
Database Links

HGNC: 16283

OMIM: 606556

KEGG: hsa:9830

STRING: 9606.ENSP00000343990

UniGene: Hs.575631

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