Recombinant Human Tyrosine-protein phosphatase non-receptor type 4 (PTPN4), partial

Code CSB-YP019041HU
MSDS
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Source Yeast
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Code CSB-EP019041HU
MSDS
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Source E.coli
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Code CSB-EP019041HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019041HU
MSDS
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Source Baculovirus
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Code CSB-MP019041HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PTPN4
Uniprot No.
Alternative Names
MEG; megakaryocyte phosphatase; megakaryocyte protein-tyrosine phosphatase; Protein tyrosine phosphatase MEG1; Protein tyrosine phosphatase; megakaryocyte; protein tyrosine phosphatase; non-receptor type 4 (megakaryocyte); Protein-tyrosine phosphatase MEG1; PTN4; PTN4_HUMAN; PTPase MEG1; PTPase-MEG1; PTPMEG; PTPMEG1; Ptpn4; Tyrosine protein phosphatase non receptor type 4; Tyrosine-protein phosphatase non-receptor type 4
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis. Regulates neuronal cell homeostasis by protecting neurons against apoptosis. Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction. Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway. May act at junctions between the membrane and the cytoskeleton.
Gene References into Functions
  1. Binding assays confirmed that three (Cyto8-mtxd-1, Cyto8-mtxk-4 and Cyto8-mtxd-1k-4) out of the five designed peptides exhibit moderately or considerably increased affinity as compared to the native peptide Cyto8-RETEV PMID: 27923202
  2. the p38gamma.PTPN4 interaction promotes cellular signaling, preventing cell death induction. PMID: 27246854
  3. Deletion of PTPN4 has been reported in monozygotic twins with a Rett syndrome-like phenotype. PMID: 25424712
  4. The physiologically active PTPN4 two-domain (the PDZ and the phosphatase domains) adopts a predominant compact conformation in solution. PDZ ligand binding restores the catalytic competence of PTPN4 disrupting the transient interdomain communication. PMID: 25158884
  5. These findings suggest that PTPN4 negatively regulates cell proliferation and motility through dephosphorylation of CrkI. PMID: 23666597
  6. PTPN4 is dispensable for T cell development and/or T cell effector functions. PMID: 18614237

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cytoplasm.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class subfamily
Database Links

HGNC: 9656

OMIM: 176878

KEGG: hsa:5775

STRING: 9606.ENSP00000263708

UniGene: Hs.469809

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