Recombinant Human Tyrosine--tRNA ligase, mitochondrial (YARS2)

Code CSB-YP896882HU
MSDS
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Source Yeast
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Code CSB-EP896882HU
MSDS
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Source E.coli
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Code CSB-EP896882HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896882HU
MSDS
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Source Baculovirus
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Code CSB-MP896882HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YARS2
Uniprot No.
Alternative Names
CGI 04; FLJ13995; mitochondrial; MLASA2; mt TyrRS; SYYM_HUMAN; Tyrosine tRNA ligase 2 mitochondrial; Tyrosine tRNA ligase; Tyrosine tRNA ligase mitochondrial; Tyrosine--tRNA ligase; Tyrosyl tRNA synthetase 2 mitochondrial; Tyrosyl tRNA synthetase mitochondrial precursor; Tyrosyl-tRNA synthetase; TyrRS; YARS 2; YARS2
Species
Homo sapiens (Human)
Expression Region
17-477
Target Protein Sequence
TLNL SVLLPLGLRK AHSGAQGLLA AQKARGLFKD FFPETGTKIE LPELFDRGTA SFPQTIYCGF DPTADSLHVG HLLALLGLFH LQRAGHNVIA LVGGATARLG DPSGRTKERE ALETERVRAN ARALRLGLEA LAANHQQLFT DGRSWGSFTV LDNSAWYQKQ HLVDFLAAVG GHFRMGTLLS RQSVQLRLKS PEGMSLAEFF YQVLQAYDFY YLFQRYGCRV QLGGSDQLGN IMSGYEFINK LTGEDVFGIT VPLITSTTGA KLGKSAGNAV WLNRDKTSPF ELYQFFVRQP DDSVERYLKL FTFLPLPEID HIMQLHVKEP ERRGPQKRLA AEVTKLVHGR EGLDSAKRCT QALYHSSIDA LEVMSDQELK ELFKEAPFSE FFLDPGTSVL DTCRKANAIP DGPRGYRMIT EGGVSINHQQ VTNPESVLIV GQHILKNGLS LLKIGKRNFY IIKWLQL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
Gene References into Functions
  1. The p.Leu392Ser variant is likely a newly identified founder YARS2 mutation in mitochondrial myopathy. PMID: 28395030
  2. The mutation in YARS2 gene is a nuclear modifier for the phenotypic manifestation of Leber's hereditary optic neuropathy-associated mitochondrial DNA mutation. PMID: 26647310
  3. Data identified novel YARS2 mutations and noted marked phenotypic variability among YARS2 MLASA patients, with phenotypes ranging from mild to lethal, suggesting that the background mtDNA haplotype may be contributing to the phenotypic variability. PMID: 24344687
  4. The study confirms mutations in YARS2 as a cause of MLASA and shows that, like some of the cytoplasmic ARSs, mitochondrial ARSs occur in high-molecular-weight complexes. PMID: 22504945
  5. first example of a TyrRS lacking specificity toward N1-N72 and thus of a TyrRS disobeying the identity rules PMID: 15840810
  6. The YARS2 mutation reported here is an alternative cause of MLASA. PMID: 20598274
  7. The gene for mitochondrial tyrosyl-tRNA synthetase is described and the initial characterization of the enzyme is reported. Genes for the remaining missing synthetases have also been found with the exception of human glutaminyl-tRNA synthetase. PMID: 15779907
  8. the structure of a strictly mitochondrial human synthetase, namely tyrosyl-tRNA synthetase (mt-TyrRS), in complex with an adenylate analog at 2.2 A resolution PMID: 17997975
  9. the apparent occurrence of an unusual TG 3' splice site in intron 5 is discussed PMID: 17672918

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Involvement in disease
Myopathy with lactic acidosis and sideroblastic anemia 2 (MLASA2)
Subcellular Location
Mitochondrion matrix.
Protein Families
Class-I aminoacyl-tRNA synthetase family
Database Links

HGNC: 24249

OMIM: 610957

KEGG: hsa:51067

STRING: 9606.ENSP00000320658

UniGene: Hs.505231

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