Recombinant Human UAP56-interacting factor (FYTTD1)

Code CSB-YP842744HU
MSDS
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Source Yeast
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Code CSB-EP842744HU
MSDS
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Source E.coli
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Code CSB-EP842744HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842744HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP842744HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FYTTD1
Uniprot No.
Alternative Names
DKFZp761B1514; Forty two three domain containing 1; Forty two three domain containing protein 1; Forty-two-three domain-containing protein 1; FYTTD 1; fyttd1; OTTHUMP00000208028; OTTHUMP00000208031; OTTHUMP00000208032; Protein 40 2 3; Protein 40-2-3; UAP56 interacting factor; UAP56-interacting factor; UIF; UIF_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-318
Target Protein Sequence
MNRFGTRLVG ATATSSPPPK ARSNENLDKI DMSLDDIIKL NRKEGKKQNF PRLNRRLLQQ SGAQQFRMRV RWGIQQNSGF GKTSLNRRGR VMPGKRRPNG VITGLAARKT TGIRKGISPM NRPPLSDKNI EQYFPVLKRK ANLLRQNEGQ RKPVAVLKRP SQLSRKNNIP ANFTRSGNKL NHQKDTRQAT FLFRRGLKVQ AQLNTEQLLD DVVAKRTRQW RTSTTNGGIL TVSIDNPGAV QCPVTQKPRL TRTAVPSFLT KREQSDVKKV PKGVPLQFDI NSVGKQTGMT LNERFGILKE QRATLTYNKG GSRFVTVG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Could you please confirm whether you have tested the activity of this protein?
http://www.cusabio.com/Recombinant-Protein/Recombinant-Human-UAP56-interacting-factorFYTTD1-Baculovirus-12425976.html
If so, can you please provide details of the assay used? And the results?

A:
Thanks for your inquiry. For this protein's activity, we haven't detected it. It's a mature full length protein, theoretically it have activity. Pls refer to http://www.jbc.org/content/282/31/22544.short
Here we attach the product details for your reference:
Recombinant Human UAP56-interacting factor(FYTTD1)
CSB-BP842744HU >> Baculovirus .
Express region:1-318aa;Full length protein.
Tag information: N terminal 10xHis-tagged + C terminal Myc-tagged
Target Protein Sequence:

MNRFGTRLVGATATSSPPPKARSNENLDKIDMSLDDIIKLNRKEGKKQNFPRLNRRLLQQSGAQQFRMRVRWGIQQNSGFGKTSLNRRGRVMPGKRRPNGVITGLAARKTTGIRKGISPMNRPPLSDKNIEQYFPVLKRKANLLRQNEGQRKPVAVLKRPSQLSRKNNIPANFTRSGNKLNHQKDTRQATFLFRRGLKVQAQLNTEQLLDDVVAKRTRQWRTSTTNGGILTVSIDNPGAVQCPVTQKPRLTRTAVPSFLTKREQSDVKKVPKGVPLQFDINSVGKQTGMTLNERFGILKEQRATLTYNKGGSRFVTVG


Target Background

Function
Required for mRNA export from the nucleus to the cytoplasm. Acts as an adapter that uses the DDX39B/UAP56-NFX1 pathway to ensure efficient mRNA export and delivering to the nuclear pore. Associates with spliced and unspliced mRNAs simultaneously with ALYREF/THOC4.
Gene References into Functions
  1. FYTTD1 was named UIF for UAP56 Interacting Factor. UIF is an mRNA export adaptor, recruited to mRNA by SSRP1 of the FACT complex. It also binds mRNA and NXF1. It is required for efficient nuclear export of mRNA in vertebrates and other animals. PMID: 19836239
Subcellular Location
Nucleus, nucleoplasm. Nucleus speckle.
Protein Families
UIF family
Tissue Specificity
Expressed in a wide variety of cancer types.
Database Links

HGNC: 25407

OMIM: 616933

KEGG: hsa:84248

STRING: 9606.ENSP00000241502

UniGene: Hs.277533

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