Recombinant Human UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2), partial

Code CSB-YP822760HU
MSDS
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Source Yeast
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Code CSB-EP822760HU
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Source E.coli
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Code CSB-EP822760HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP822760HU
MSDS
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Source Baculovirus
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Code CSB-MP822760HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
B3GALNT2
Uniprot No.
Alternative Names
3-GalNAc-T2; 3-N-acetylgalactosaminyltransferase 2; 3-N-acetylgalactosaminyltransferase II; B3GalNAc T2; B3GalNAcT2; b3galnt2; B3GL2_HUMAN; Beta 1,3 galactosaminyltransferase 2; Beta 1,3 N acetylgalactosaminyltransferase 2; Beta 1,3 N acetylgalactosaminyltransferase II; Beta 1,3 N acetylgalactosaminyltransferase II MGC39558; Beta 3 GalNAc T2; Beta-1; beta-1,3-GalNAc-T2; Beta3GalNAcT2; MDDGA11; MGC39558; UDP GalNAc:betaGlcNAc beta 1,3 galactosaminyltransferase polypeptide 2; UDP-GalNAc:beta-1; UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Beta-1,3-N-acetylgalactosaminyltransferase that synthesizes a unique carbohydrate structure, GalNAc-beta-1-3GlcNAc, on N- and O-glycans. Has no galactose nor galactosaminyl transferase activity toward any acceptor substrate. Involved in alpha-dystroglycan (DAG1) glycosylation: acts coordinately with GTDC2/POMGnT2 to synthesize a GalNAc-beta3-GlcNAc-beta-terminus at the 4-position of protein O-mannose in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan, which is required for binding laminin G-like domain-containing extracellular proteins with high affinity.
Gene References into Functions
  1. Mutations in B3GALNT2 give rise to a novel muscular dystrophy-dystroglycanopathies syndrome presentation, characterized by intellectual disability and seizure, but without any apparent muscular involvement. PMID: 29273094
  2. B3GALNT2 is a gene associated with congenital muscular dystrophy with brain malformations. PMID: 24084573
  3. B3GALNT2 overexpression is associated with breast cancer. PMID: 24285400
  4. Results demonstrate a role for B3GALNT2 in the glycosylation of alpha-DG and show that B3GALNT2 mutations can cause dystroglycanopathy with muscle and brain involvement. PMID: 23453667
  5. Although the GalNAcbeta1-3GlcNAcbeta1-R structure has not been reported in humans or other mammals, we have discovered a novel human glycosyltransferase producing this structure on N- and O-glycans. PMID: 14724282

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Involvement in disease
Muscular dystrophy-dystroglycanopathy congenital with brain and eye anomalies A11 (MDDGA11)
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein. Endoplasmic reticulum.
Protein Families
Glycosyltransferase 31 family
Tissue Specificity
Expressed in all tissues examined, but at highest levels in testis, adipose tissue, skeletal muscle and ovary.
Database Links

HGNC: 28596

OMIM: 610194

KEGG: hsa:148789

STRING: 9606.ENSP00000355559

UniGene: Hs.498143

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