Recombinant Human UMP-CMP kinase (CMPK1)

Code CSB-YP005616HU
MSDS
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Source Yeast
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Code CSB-EP005616HU
MSDS
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Source E.coli
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Code CSB-EP005616HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005616HU
MSDS
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Source Baculovirus
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Code CSB-MP005616HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
CMK; CMPK 1; CMPK; cmpk1; Cytidine monophosphate (UMP CMP) kinase 1 cytosolic; Cytidine monophosphate kinase; Cytidine monophosphate UMP CMP kinase 1 cytosolic; Cytidylate kinase; dCMP kinase; Deoxycytidylate kinase; EC 2.7.4.14; KCY; KCY_HUMAN; Monophosphatase kinase; OTTHUMP00000009565; RP11 511I2.1; UCK; UMK; UMP CMP; UMP CMP kinase; UMP-CMP kinase; UMP/CMP kinase; UMP/CMPK; UMPK; Uridine monophosphate kinase; Uridine monophosphate/cytidine monophosphate kinase
Species
Homo sapiens (Human)
Expression Region
1-196
Target Protein Sequence
MKPLVVFVLG GPGAGKGTQC ARIVEKYGYT HLSAGELLRD ERKNPDSQYG ELIEKYIKEG KIVPVEITIS LLKREMDQTM AANAQKNKFL IDGFPRNQDN LQGWNKTMDG KADVSFVLFF DCNNEICIER CLERGKSSGR SDDNRESLEK RIQTYLQSTK PIIDLYEEMG KVKKIDASKS VDEVFDEVVQ IFDKEG
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.
Gene References into Functions
  1. Nuclear CMPK1 expression is indicative of poor prognosis in the triple negative breast cancer patients. PMID: 27558661
  2. TGF-b1 inhibited the expression of CMPK, which was blocked in the presence of a TGF-b type I receptor, SB431542, and was abolished by the inhibitor of miR-130b-3p, indicating that CMPK is regulated by the TGF-b signalling pathway through the upregulation of miR-130b-3p. PMID: 28414100
  3. The role of CMP/UMP kinase (CMPK), an enzyme catalyzes cytidine diphosphate formation, in DNA repair, was investigated. PMID: 25659034
  4. CMPK1 and RBP3 are associated with corneal curvature in Asian populations. PMID: 24963161
  5. In particular, polymorphisms of the CMPK1 gene seem to provide important prognostic information PMID: 22838950
  6. Single-nucleotide polymorphisms in CMPK1 gene is associated with treatment response in non-small cell lung cancer. PMID: 21642870
  7. UMP/CMP kinase (EC 2.7.4.14) is not critical for the phosphorylation of CMP, dCMP and maintenance of natural nucleotide pools in cells PMID: 21559290
  8. thymidylate synthase/ribonucleotide reductase gene silencing and deoxycytidine kinase::uridine monophosphate kinase fusion gene gene overexpression markedly improved gemcitabine's therapeutic activity PMID: 19568409
  9. Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates. PMID: 11912132
  10. UMP/CMP kinase reveals the conformational changes that occur upon substrate binding PMID: 15163660
  11. Models for the phosphorylation action and substrate specificity of human UMP/CMP kinase. PMID: 15550676
  12. Several uracil vinylphosphonate derivatives had affinities for human UMP-CMP kinase similar to those of dUMP PMID: 17608725

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Adenylate kinase family, UMP-CMP kinase subfamily
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 18170

OMIM: 191710

KEGG: hsa:51727

STRING: 9606.ENSP00000360939

UniGene: Hs.731647

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