Recombinant Human Uncharacterized protein KIAA0226-like (KIAA0226L)

Code CSB-YP884481HU
MSDS
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Source Yeast
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Code CSB-EP884481HU
MSDS
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Source E.coli
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Code CSB-EP884481HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884481HU
MSDS
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Source Baculovirus
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Code CSB-MP884481HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RUBCNL
Uniprot No.
Alternative Names
C13orf18; CM018_HUMAN; FLJ21562; FLJ43762 ; KIAA0226 like; KIAA0226L; RP11-247M1.4; Uncharacterized protein C13orf18; Uncharacterized protein KIAA0226-like
Species
Homo sapiens (Human)
Expression Region
1-662
Target Protein Sequence
MVSQSTVRQD SPVEPWEGIS DHSGIIDGSP RLLNTDHPPC QLDIRLMRHK AVWINPQDVQ QQPQDLQSQV PAAGNSGTHF VTDAASPSGP SPSCLGDSLA ETTLSEDTTD SVGSASPHGS SEKSSSFSLS STEVHMVRPG YSHRVSLPTS PGILATSPYP ETDSAFFEPS HLTSAADEGA VQVSRRTISS NSFSPEVFVL PVDVEKENAH FYVADMIISA MEKMKCNILS QQQTESWSKE VSGLLGSDQP DSEMTFDTNI KQESGSSTSS YSGYEGCAVL QVSPVTETRT YHDVKEICKC DVDEFVILEL GDFNDITETC SCSCSSSKSV TYEPDFNSAE LLAKELYRVF QKCWILSVVN SQLAGSLSAA GSIVVNEECV RKDFESSMNV VQEIKFKSRI RGTEDWAPPR FQIIFNIHPP LKRDLVVAAQ NFFCAGCGTP VEPKFVKRLR YCEYLGKYFC DCCHSYAESC IPARILMMWD FKKYYVSNFS KQLLDSIWHQ PIFNLLSIGQ SLYAKAKELD RVKEIQEQLF HIKKLLKTCR FANSALKEFE QVPGHLTDEL HLFSLEDLVR IKKGLLAPLL KDILKASLAH VAGCELCQGK GFICEFCQNT TVIFPFQTAT CRRCSACRAC FHKQCFQSSE CPRCARITAR RKLLESVASA AT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulator of autophagy that promotes autophagosome maturation by facilitating the biogenesis of phosphatidylinositol 3-phosphate (PtdIns(3)P) in late steps of autophagy. Acts by antagonizing RUBCN, thereby stimulating phosphatidylinositol 3-kinase activity of the PI3K/PI3KC3 complex. Following anchorage to the autophagosomal SNARE STX17, promotes the recruitment of PI3K/PI3KC3 and HOPS complexes to the autophagosome to regulate the fusion specificity of autophagosomes with late endosomes/lysosomes. Binds phosphoinositides phosphatidylinositol 3-phosphate (PtdIns(3)P), 4-phosphate (PtdIns(4)P) and 5-phosphate (PtdIns(5)P). In addition to its role in autophagy, acts as a regulator of lipid and glycogen homeostasis. May act as a tumor suppressor.
Gene References into Functions
  1. Pacer localizes to autophagic structures and positively regulates autophagosome maturation. PMID: 28306502
  2. Re-activation of C13ORF18 led to partial demethylation of the C13ORF18 promoter and decreased repressive histone methylation. PMID: 23522960
  3. Data suggest that the four-gene methylation panel might provide an alternative triage test after primary high-risk papillomavirus (hr-HPV) testing. PMID: 21796628
  4. Methylation of C13orf18 in cervical scrapings is strongly associated with high-grade cervical intraepithelial neoplasia and cervical cancer. PMID: 19843677
Subcellular Location
Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein.
Tissue Specificity
Expressed weakly in cervical carcinoma cell lines.
Database Links

HGNC: 20420

KEGG: hsa:80183

STRING: 9606.ENSP00000374558

UniGene: Hs.98117

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