Recombinant Human Voltage-dependent R-type calcium channel subunit alpha-1E (CACNA1E), partial

Code CSB-YP623017HU
MSDS
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Source Yeast
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Code CSB-EP623017HU
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Source E.coli
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Code CSB-EP623017HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP623017HU
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Source Baculovirus
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Code CSB-MP623017HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CACNA1E
Uniprot No.
Alternative Names
alpha-1 polypeptide; BII; Brain calcium channel II; CAC 1E; CAC1E; CAC1E_HUMAN; CACH 6; CACH6; CACNA1E; Cacnl1 a6; CACNL1A6; Calcium channel; Calcium channel; L type; alpha 1 polypeptide; isoform 6; calcium channel; R type; Cav 2.3; Cchra 1; Cchra1; isoform 6; L type; Voltage dependent R type calcium channel subunit alpha 1E; Voltage gated calcium channel subunit alpha Cav2.3; Voltage-dependent R-type calcium channel subunit alpha-1E; Voltage-gated calcium channel subunit alpha Cav2.3
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells. They are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel. They are however insensitive to dihydropyridines (DHP). Calcium channels containing alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.
Gene References into Functions
  1. This study identified a polymorphism in exon 20 of the CACNA1E gene (Asp859Glu - rs35737760) that is more prevalent in hemiplegic and brain stem aura migraine. PMID: 28573794
  2. Carriers of the minor G allele of the rs3845446 SNP exhibited enhanced pain-related phenotypes after gastrointestinal surgery. The pain-related phenotypes of carriers of the minor allele of this SNP after gastrointestinal surgery were opposite to such phenotypes after orthognathic surgery. PMID: 27480382
  3. Data suggest that, in vascular smooth muscle cells, increase in cytosolic endothelin-1 (EDN1) induces sustained increase in nuclear Ca2+ via stimulation of R-type calcium channel (CACNA1E) present at the nuclear membrane. PMID: 25741585
  4. CACNA1E gene single nucleotide polymorphisms may be involved in fentanyl sensitivity. PMID: 23940630
  5. The C-terminus of human Ca(v)2.3 voltage-gated calcium channel interacts with alternatively spliced calmodulin-2 expressed in two human cell lines PMID: 22633975
  6. quartet of leucine residues in the guanylate kinase domain of CaVbeta determines the plasma membrane density of the CaV2.3 channel. PMID: 22846999
  7. CACNA1E variants affect beta cell function in patients with newly diagnosed type 2 diabetes. PMID: 22427875
  8. the II-III loop of the Ca(v)2.3 calcium channel binds APLP1 and that this binding promotes internalization of the channel PMID: 22178872
  9. Ca(v)2.3 Ca2+ channel interacts with the G1-subunit of V-ATPase. PMID: 21691059
  10. Lack of high-voltage activated Cav2.3 channels results in a marked decrease in the sensitivity of transgenic animals to gamma-butyrolactone-induced absence epilepsy. PMID: 21482359
  11. Swapping the I-II intracellular linker between L-type CaV1.2 and R-type CaV2.3 high-voltage gated calcium channels exchanges activation attributes. PMID: 20026913
  12. Review discusses the roles of Cav2.3-containing E-type Ca2+ channels which exhibit several subunit-specific features, trigger exocytosis and are also involved in long-term potentiation. PMID: 15845089
  13. Full-length RGS3, RGS3T, and the core domain of RGS3 were equally effective in antagonizing inhibition of Ca(V)2.3 through M(2)R. PMID: 16855219
  14. Neurokinin 1 receptorsmodulate CaV2.3 using three different signaling mechanisms: a fast inhibition mediated by Gbeta gamma, a slow inhibition mediated by Galpha(q/11), and a slow stimulation mediated by protein kinases C. PMID: 17050807
  15. Amplification and overexpression of CACNA1E correlates with relapse in favorable histology Wilms' tumors. PMID: 17189400
  16. analysis of a three-dimensional homology model of Ca(V)2.3 based upon Kv1.2 where hydrophobic residues at positions facing Val(1720) in IS6, IIS6, and IIIS6 play a critical role in stabilizing the closed state in Ca(V)2.3 PMID: 17660294
  17. A functional variant in CACNA1E contributes to type 2 diabetes susceptibility in Pima indians by affecting insulin action. PMID: 17720895
  18. Genetic variation in the CACNA1E gene contributes to an increased risk of the development of type 2 diabetes by reducing insulin secretion. PMID: 17934712
  19. These findings strongly suggest that both H179 and H183 in the IS3-IS4 loop are essential structural determinants required for nickel sensitive inhibition of the Cav2.3. PMID: 18037383

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Calcium channel alpha-1 subunit (TC 1.A.1.11) family, CACNA1E subfamily
Tissue Specificity
Expressed in neuronal tissues and in kidney.
Database Links

HGNC: 1392

OMIM: 601013

KEGG: hsa:777

STRING: 9606.ENSP00000356545

UniGene: Hs.437444

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