Recombinant Human WD repeat-containing protein 46(WDR46)

Code CSB-YP026034HU
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Source Yeast
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Code CSB-EP026034HU
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Source E.coli
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Code CSB-EP026034HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026034HU
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Source Baculovirus
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Code CSB-MP026034HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names WDR46
Uniprot No. O15213
Alternative Names WDR46; BING4; C6orf11; FP221; WD repeat-containing protein 46; WD repeat-containing protein BING4
Species Homo sapiens (Human)
Expression Region 1-610
Target Protein Sequence METAPKPGKD VPPKKDKLQT KRKKPRRYWE EETVPTTAGA SPGPPRNKKN RELRPQRPKN AYILKKSRIS KKPQVPKKPR EWKNPESQRG LSGTQDPFPG PAPVPVEVVQ KFCRIDKSRK LPHSKAKTRS RLEVAEAEEE ETSIKAARSE LLLAEEPGFL EGEDGEDTAK ICQADIVEAV DIASAAKHFD LNLRQFGPYR LNYSRTGRHL AFGGRRGHVA ALDWVTKKLM CEINVMEAVR DIRFLHSEAL LAVAQNRWLH IYDNQGIELH CIRRCDRVTR LEFLPFHFLL ATASETGFLT YLDVSVGKIV AALNARAGRL DVMSQNPYNA VIHLGHSNGT VSLWSPAMKE PLAKILCHRG GVRAVAVDST GTYMATSGLD HQLKIFDLRG TYQPLSTRTL PHGAGHLAFS QRGLLVAGMG DVVNIWAGQG KASPPSLEQP YLTHRLSGPV HGLQFCPFED VLGVGHTGGI TSMLVPGAGE PNFDGLESNP YRSRKQRQEW EVKALLEKVP AELICLDPRA LAEVDVISLE QGKKEQIERL GYDPQAKAPF QPKPKQKGRS STASLVKRKR KVMDEEHRDK VRQSLQQQHH KEAKAKPTGA RPSALDRFVR
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Scaffold component of the nucleolar structure. Required for localization of DDX21 and NCL to the granular compartment of the nucleolus.
Gene References into Functions
  1. When WDR46 was knocked down, nucleolin and DDX21 were mislocalized from the granular component to the edges of the nucleoli. PMID: 23848194
Subcellular Location Nucleus, nucleolus.
Database Links

HGNC: 13923

OMIM: 611440

KEGG: hsa:9277

STRING: 9606.ENSP00000363746

UniGene: Hs.520063

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Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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