Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

UL123

Uniprot No.

F5HCM1

Research Area

Others

Species

Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)

Source

E.coli

Expression Region

1-491aa

Target Protein Sequence

MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFPELAEESLKTFEQVTEDCNENPEKDVLTELVKQIKVRVDMVRHRIKEHMLKKYTQTEEKFTGAFNMMGGCLQNALDILDKVHEPFEDMKCIGLTMQSMYENYIVPEDKREMWMACIKELHDVSKGAANKLGGALQAKARAKKDELRRKMMYMCYRNIEFFTKNSAFPKTTNGCSQAMAALQNLPQCSPDEIMSYAQKIFKILDEERDKVLTHIDHIFMDILTTCVETMCNEYKVTSDACMMTMYGGISLLSEFCRVLCCYVLEETSVMLAKRPLITKPEVISVMKRRIEEICMKVFAQYILGADPLRVCSPSVDDLRAIAEESDEEEAIVAYTLATAGASSSDSLVSPPESPVPATIPLSSVIVAENSDQEESEQSDEEQEEGAQEEREDTVSVKSEPVSEIEEVASEEEEDGAEEPTASGGKSTHPMVTRSKADQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

62.6 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Plays an important role in transactivating viral early genes as well as activating its own promoter, probably by altering the viral chromatin structure. Expression of IE1 and IE2 proteins is critical for the establishment of lytic infection and reactivation from viral latency. Disrupts PML-associated ND10 nuclear bodies by interfering with host PML and SP100 sumoylation thereby altering the regulation of type I and type II interferon-induced gene expression. Promotes efficient viral growth by interacting with and directing host SP100 to degradation, leading to enhanced acetylation level of histones. In addition, functions in counteracting the host innate antiviral response. Inhibits the type I interferon pathway by directly interacting with and sequestrating host STAT2. Also targets type II interferon pathway by repressing IL6- and STAT3 target genes. Repression of STAT3 genes is due to STAT3 nuclear accumulation and disruption of IL6-induced STAT3 phosphorylation by IE1. This repression is followed by phosphorylation and activation of STAT1. Inhibits host ISG transcription by sequestering host ISGF3 in a PML- and STAT2- binding dependent manner. Alters host cell cycle progression, probably through its interaction with host E2F1 or RB1 that overcomes the RB1-mediated repression of E2F-responsive promoters.

Gene References into Functions

Human cytomegalovirus IE1 causes SOX2 downregulation by promoting the nuclear accumulation and inhibiting the phosphorylation of STAT3, a transcriptional activator of SOX2 expression. PMID: 29950413
IE1 is involved in Hes1 degradation by assembling a ubiquitination complex and promoting Hes1 ubiquitination as a potential E3 ubiquitin ligase, followed by proteasomal degradation of Hes1. PMID: 28750047
IE2 disrupts the orderly process of brain development in a stepwise manner, furthering our understanding of neurodevelopmental human cytomegalovirus pathogenesis PMID: 28615204
data reveal that MICA and PVR are directly regulated by human cytomegalovirus immediate early proteins, and this may be crucial for the onset of an early host antiviral response PMID: 27733551
findings demonstrate that the PML protein, which mediates an intrinsic immune response against human cytomegalovirus, specifically serves as an E3 ligase for SUMO modification of IE1p72 PMID: 28250117
we present transcriptome data indicating that IE1 is as significant a repressor as it is an activator of host gene expression PMID: 27387064
These data suggest that targeting PML by human herpesvirus 5 IE1 represents a strategy to antagonize both intrinsic and innate immune mechanisms. PMID: 26559840
co-immunoprecipitation experiments demonstrate that IE1CORE binds via the coiled-coil domain to PML and also interacts with TRIM5alpha PMID: 25412268
IE1- and pp71 mediated viral strategies against cellular defenses.[review] PMID: 25501994
Human cytomegalovirus IE1 exon 4 interacts with Topoisomerase IIbeta (TOPOIIbeta), whose activity is required for viral genome persistence and maintenance via binding to a cis-acting viral maintenance element. PMID: 25011107
IE1 and IE2 are required for efficient human cytomegalovirus gene expression. PMID: 25552717
Together, these results indicate that cytomegalovirus IE1 disrupts IFNgamma signaling by interfering with signaling events in the nucleus through a novel mechanism. PMID: 24699362
GST pulldown experiments revealed that both IE72 and wt p53 bound the important homology-directed repair protein, Rad51. PMID: 24576846
Antisense transcripts of UL123 were packaged in highly purified virions. PMID: 23884634
IE1 protein targets host chromosomes by docking to the acidic pocket on the nucleosome surface. PMID: 24227840
pp71 stimulates major histocompatibility complex class i presentation of IE1-derived peptides at immediate early times of infection. PMID: 23449799
These results suggest that the effects of cytomegalovirus IE1 and pp71 on subsets of ND10 components combine to mirror the overall activities of herpes simplex virus 1 ICP0. PMID: 23135716
Human cytomegalovirus IE1/2 expression was downregulated by cyclin A2, CDK1 and CDK2. PMID: 22718829
chromatin-tethering domain required for associations of IE1, PML and STAT2 with mitotic chromosomes, but not essential for viral replication PMID: 22158879
These findings indicate that thehuman herpesvirus 5 IE1-dependent loss of human Sp100 proteins during virus infection may represent an important requirement for efficient viral growth. PMID: 21880768

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Subcellular Location

Host nucleus.

Protein Families

HHV-5 IE1 protein family

Database Links

KEGG: vg:3077513

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Code	CSB-EP522280HWW
MSDS	MSDS
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human cytomegalovirus Immediate early protein IE1 (UL123)

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