Product Details

Purity

>85% (SDS-PAGE)

Target Names

HELI

Uniprot No.

P10189

Alternative Names

HELI; UL5DNA replication helicase; EC 3.6.4.-

Species

Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Possesses helicase-like motifs and therefore may act as the helicase subunit of the complex.

Gene References into Functions

Data indicate that the N-terminal domain of UL52 primase binds UL5 helicase and the middle domain interacts with Ul8. PMID: 25278021
Results showed that the UL29 and UL5 transgenes are stable in that there are one and three copies of the UL29 and UL5 genes, respectively, per cell in both the AV529-19 MCB and ECB. PMID: 24140636
HSV-1 single stranded DNA binding protein (ICP8), and the helicase/primase complex (UL8/UL5/UL52) form a nuclear complex in transfected cells that is necessary and sufficient to disable ATR signaling. PMID: 24098119
Taken together, these data suggest that the primase activity of the helicase-primase requires formation of a dimer or higher-order structure while ATPase activity does not. PMID: 21068246
We present evidence that single mutations close to a predicted functional domain of an essential HSV-1 replication enzyme (helicase) are associated with drug resistance and virus growth characteristics. PMID: 17404685
We believe our report to be the first to describe an HPI(helicase-primase inhibitors)-resistant HSV-1 UL5 mutant, that is markedly less virulent in vivo and slowly growing in tissue culture compared to the parental strain. [UL5] PMID: 18539344
Slower growth and moderately reduced virulence suggest that the Asn342Lys mutation might interferes with the helicase-primase activity. PMID: 19403757
Herpes simplex virus-1 DNA primase does not absolutely need to form Watson-Crick hydrogen bonds to efficiently polymerize a NTP. The enzyme can misincorporate nucleotides both by misreading the template and by a primer-template slippage mechanism. PMID: 19835416

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Subcellular Location

Host nucleus.

Protein Families

Herpesviridae helicase family

Database Links

KEGG: vg:2703420

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Recombinant Human herpesvirus 1 Probable ATP-dependent helicase UL5 (UL5), partial

Product Details

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Target Background

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