Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat

Product Type Recombinant Protein
Uniprot No. P19553
Species Human immunodeficiency virus type 1 group M subtype B (isolate SF162) (HIV-1)
Gene Names tat
Protein Names Recommended name: Protein Tat Alternative name(s): Transactivating regulatory protein
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Nuclear transcriptional activator of viral gene expression, that is essential for viral transcription from the LTR promoter and replication. Acts as a sequence-specific molecular adapter, directing components of the cellular transcription machinery to the viral RNA to promote processive transcription elongation by the RNA polymerase II (RNA pol II) complex, thereby increasing the level of full-length transcripts. In the absence of Tat, the RNA Pol II generates short or non-processive transcripts that terminate at approximately 60 bp from the initiation site. Tat associates with the CCNT1/cyclin-T1 component of the P-TEFb complex (CDK9 and CCNT1), which promotes RNA chain elongation. This binding increases Tat's affinity for a hairpin structure at the 5'-end of all nascent viral mRNAs referred to as the transactivation responsive RNA element (TAR RNA) and allows Tat/P-TEFb complex to bind cooperatively to TAR RNA. The CDK9 component of P-TEFb and other Tat-activated kinases hyperphosphorylate the C-terminus of RNA Pol II that becomes stabilized and much more processive. Other factors such as HTATSF1/Tat-SF1, SUPT5H/SPT5, and HTATIP2 are also important for Tat's function. Besides its effect on RNA Pol II processivity, Tat induces chromatin remodeling of proviral genes by recruiting the histone acetyltransferases (HATs) CREBBP, EP300 and PCAF to the chromatin. This also contributes to the increase in proviral transcription rate, especially when the provirus integrates in transcriptionally silent region of the host genome. To ensure maximal activation of the LTR, Tat mediates nuclear translocation of NF-kappa-B by interacting with host RELA. Through its interaction with host TBP, Tat may also modulate transcription initiation. Tat can reactivate a latently infected cell by penetrating in it and transactivating its LTR promoter. In the cytoplasm, Tat is thought to act as a translational activator of HIV-1 mRNAs.
Subcellular Location Host nucleus, host nucleolus, Host cytoplasm, Secreted
Protein Families Lentiviruses Tat family

Product Details for Different Expression Systems

Product Name Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat,Yeast
Code CSB-YP322543HLD
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MEPVDPRLEP WKHPGSQPKT ACTNCYCKKC CFHCQVCFIT KGLGISYGRK KRRQRRRAPP DSEVHQVSLP KQPASQPQGD PTGPKESKKK VERETETDPV H
Expression Region 1-101
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat,E.coli
Code CSB-EP322543HLD
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MEPVDPRLEP WKHPGSQPKT ACTNCYCKKC CFHCQVCFIT KGLGISYGRK KRRQRRRAPP DSEVHQVSLP KQPASQPQGD PTGPKESKKK VERETETDPV H
Expression Region 1-101
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat,Baculovirus
Code CSB-BP322543HLD
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MEPVDPRLEP WKHPGSQPKT ACTNCYCKKC CFHCQVCFIT KGLGISYGRK KRRQRRRAPP DSEVHQVSLP KQPASQPQGD PTGPKESKKK VERETETDPV H
Expression Region 1-101
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat,Mammalian cell
Code CSB-MP322543HLD
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MEPVDPRLEP WKHPGSQPKT ACTNCYCKKC CFHCQVCFIT KGLGISYGRK KRRQRRRAPP DSEVHQVSLP KQPASQPQGD PTGPKESKKK VERETETDPV H
Expression Region 1-101
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat

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