Recombinant Human m7GpppN-mRNA hydrolase (DCP2)

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Code CSB-EP810265HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
DCP2
Uniprot No.
Research Area
Transcription
Alternative Names
DCP2; DCP2 decapping enzyme homolog (S. cerevisiae); DCP2, S. cerevisiae, homolog of; DCP2_HUMAN; decapping enzyme 2, S. cerevisiae, homolog of; decapping enzyme homolog (S. cerevisiae); FLJ33245; hDpc; m7GpppN-mRNA hydrolase; mRNA decapping enzyme 2; mRNA-decapping enzyme 2; Nucleoside diphosphate-linked moiety X motif 20; nudix (nucleoside diphosphate linked moiety X)-type motif 20; Nudix motif 20; NUDT20
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-385aa
Target Protein Sequence
METKRVEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSTGSTPAKPTVEKLSRTKFRHSQQLFPDGSPGDQWVKHRQPLQQKPYNNHSEMSDLLKGKKCEKKLHPRKLQDNFETDAVYDLPSSSEDQLLEHAEGQPVACNGHCKFPFSSRAFLSFKFDHNAIMKILDL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
48.4kDa
Protein Length
Full Length of Isoform 2
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Amino acids 1-385 form the expressed segment for recombinant Human DCP2. This DCP2 protein is theoretically predicted to have a molecular weight of 48.4 kDa. The DCP2 protein was expressed in e.coli. Fusion of the N-terminal 6xHis tag into the DCP2 encoding gene fragment was conducted, allowing for easier detection and purification of the DCP2 protein in subsequent stages.

Human m7GpppN-mRNA Hydrolase (DCP2) is a critical enzyme involved in mRNA decay. Its main function includes catalyzing the removal of the 5' cap structure (m7GpppN) from mRNA, initiating degradation. DCP2 plays a pivotal role in regulating mRNA turnover and ensuring cellular RNA quality control. In molecular biology and RNA research, studying DCP2 contributes to understanding post-transcriptional gene regulation and mRNA degradation pathways. DCP2 dysfunction is implicated in neurodegenerative diseases and cancer. Investigating DCP2 provides insights into RNA metabolism, cellular homeostasis, and potential applications in therapeutic strategies targeting aberrant mRNA decay processes in various pathophysiological conditions.

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Target Background

Function
Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
Gene References into Functions
  1. Human Dcp2 levels and activity are controlled by a competition between decapping complex assembly and Dcp2 degradation. PMID: 25870104
  2. Data show that Y14 interacts directly with the decapping factor Dcp2 and the 5' cap structure of mRNAs via different but overlapping domains. PMID: 23115303
  3. an mRNA decapping enzyme demonstrated to contain intrinsic decapping activity PMID: 12218187
  4. Human Dcp2 is a catalytically active mRNA decapping enzyme that localizes to the cytoplasm PMID: 12486012
  5. hDcp2 can specifically bind to and can regulate the stability of a subset of mRNAs PMID: 18039849
  6. These data support the novel notion of the association between Ro52 with hDCP2 protein in cytoplasmic p-bodies, playing a role in mRNA metabolism in response to cellular stimulation. PMID: 18361920

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Subcellular Location
Cytoplasm, P-body. Nucleus.
Protein Families
Nudix hydrolase family, DCP2 subfamily
Tissue Specificity
Expressed in brain and testis. Not detected in heart (at protein level).
Database Links

HGNC: 24452

OMIM: 609844

KEGG: hsa:167227

STRING: 9606.ENSP00000373715

UniGene: Hs.443875

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