Recombinant Lysine-specific demethylase rbr-2 (rbr-2), partial

Code CSB-YP631522CXY
MSDS
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Source Yeast
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Code CSB-EP631522CXY
MSDS
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Source E.coli
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Code CSB-EP631522CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP631522CXY
MSDS
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Source Baculovirus
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Code CSB-MP631522CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rbr-2
Uniprot No.
Alternative Names
rbr-2; ZK593.4Lysine-specific demethylase rbr-2; Histone demethylase rbr-2; EC 1.14.11.-; Jumonji/ARID domain-containing protein rbr-2
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Required for normal longevity of the soma in a germline-dependent manner. Implicated in the epigenetic inheritance of lifespan over several generations. Involved in larval development and vulva formation.
Gene References into Functions
  1. RBR-2 is not involved in preventing H3K4me3 deposition in the P lineage, but could play a role in regulating somatic deposition of H3K4me3. PMID: 21549110
  2. findings show that mutation or RNAi depletion of rbr-2 leads to increased levels of trimethylated histone 3 lysine 4 during larval development and defects in vulva formation PMID: 17320161
Subcellular Location
Nucleus.
Protein Families
JARID1 histone demethylase family
Database Links

KEGG: cel:CELE_ZK593.4

STRING: 6239.ZK593.4

UniGene: Cel.6207

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