Recombinant MAP kinase-activating death domain protein (aex-3), partial

Code CSB-YP013309CXY
MSDS
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Source Yeast
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Code CSB-EP013309CXY
MSDS
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Source E.coli
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Code CSB-EP013309CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013309CXY
MSDS
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Source Baculovirus
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Code CSB-MP013309CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
aex-3
Uniprot No.
Alternative Names
aex-3; C02H7.3MAP kinase-activating death domain protein; Aboc; expulsion defective protein 3; Regulator of presynaptic activity aex-3
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Guanyl-nucleotide exchange factor that regulates small GTPases. Converts GDP-bound inactive form of rab-3 and cab-1 to the GTP-bound active forms. Regulator of presynaptic activity that interacts with rab-3 to regulate synaptic vesicle release. Is also a regulator of the cab-1 synaptic transmission pathway. Probably by converting rab-3 to its GTP-bound active form, plays a role in the recruitment of endophilin unc-57 to synaptic vesicles. Probably by activating rab-3 and thus regulating the trafficking of dense-core vesicles, plays a role in AVG neuron-mediated formation of the right axon tract of the ventral nerve cord. Regulates anterior body muscle contractions (aBOC) and the expulsion steps during the defecation motor program (DMP). Probably by regulating DMP, required for fatty acid uptake by intestinal cells.
Subcellular Location
Cell membrane. Cytoplasm.
Protein Families
MADD family
Tissue Specificity
Expressed in nearly all neurons.
Database Links

KEGG: cel:CELE_C02H7.3

STRING: 6239.C02H7.3a

UniGene: Cel.18148

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