Recombinant MMACHC-like protein (cblc-1)

Code CSB-YP773576CXY
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP773576CXY
MSDS
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Source E.coli
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Code CSB-EP773576CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP773576CXY
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP773576CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cblc-1
Uniprot No.
Alternative Names
cblc-1; ZK546.17; MMACHC-like protein; EC 1.16.1.-; Cobalamin deficiency protein 1; Cyanocobalamin reductase; cyanide-eliminating)
Species
Caenorhabditis elegans
Expression Region
1-272
Target Protein Sequence
MVTEMSHAES IKRVVDQKLS SHEGFESHMF KIGSYNEAVG ESSPFALPYD DSTMALLILS TPDMFDVAFR KWVVQKTMDF GSFDEVCEMV SSPIQSFLED RLEIMSEKLR KVEENFEILH DYSMTPQRRP KILMQTCGHV AGAAFYYQPC HFQEDGVTWP PAGRMGPNLK FIGLSLHPIY GGHFAFRSVL IFPNVKIPEF CEKEPRPILT ASEDVRTALE KFNYNWKDSG FRDFGNPTRR YSTTQMEFFG RPVAERWEVL RPWVDGGAKN ID
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the reductive dealkylation of cyanocobalamin to cob(II)alamin, using FAD or FMN as cofactor and NADPH as cosubstrate. Can also catalyze the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. Under anaerobic conditions cob(I)alamin is the first product; it is highly reactive and is converted to aquocob(II)alamin in the presence of oxygen. Binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.
Gene References into Functions
  1. The crystal structure of ceCblC provides insights into how architectural differences at the alpha- and beta-faces of cobalamin promote the thiol oxidase activity of ceCblC but mute it in wild-type human CblC. PMID: 28442570
  2. ability of the worm CblC to stabilize the highly reactive cob(I)alamin product of the glutathione transferase reaction PMID: 25369151
  3. These results demonstrate that cCblC is a Btrafficking chaperone of C. elegans catalyzing dealkylation and deglutathionylation via a nucleophilic displacement using the thiolate of GSH. PMID: 25256267
Subcellular Location
Cytoplasm.
Protein Families
MMACHC family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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