Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque CTGF at 1 μg/mL can bind Anti-CTGF recombinant antibody (CSB-RA006147MA2HU). The EC50 is 0.6886-0.7825 ng/mL.

Target Names

CCN2

Uniprot No.

H9FQD5

Alternative Names

Cellular communication network factor 2; Connective tissue growth factor

Species

Macaca mulatta (Rhesus macaque)

Source

Mammalian cell

Expression Region

27-349aa

Target Protein Sequence

QNCSGPCRCPAEQAPRCPAGVSLVLDGCGCCRVCAKQLGELCTERDPCDPHKGLFCDFGSPANRKIGVCTAKDGAPCIFGGTVYRSGESFQSSCKYQCTCLDGAVGCMPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDQTVVGPALAAYRLEDTFGPDPTMIRANCLVQTTEWSACSKTCGMGISTRVTNDNASCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKISKPIKFELSGCTSVKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEVMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA

Mol. Weight

36.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCN2, also known as connective tissue growth factor, orchestrates fibroblast proliferation, extracellular matrix remodeling, and angiogenesis across wound healing and fibrotic disease models. This full-length mature protein (aa 27–349) demonstrates robust antibody-binding activity with an EC50 of 0.69–0.78 ng/mL in functional ELISA, providing a quantitative benchmark for antibody characterization studies, receptor-ligand competition assays, and ELISA standard curve generation. Mammalian cell expression preserves the native glycosylation and disulfide architecture critical for CCN2's interaction with integrins and heparan sulfate proteoglycans, supporting its use in cell proliferation assays, osteogenic and chondrogenic differentiation protocols, and angiogenesis models where post-translational fidelity directly influences biological readout. Purity exceeding 95% by SDS-PAGE and endotoxin below 1.0 EU/μg align with standards expected in primary cell culture systems and in vivo regenerative medicine studies where contaminant sensitivity is high.

Code	CSB-MP5112MOW
Abbreviation	Recombinant Rhesus macaque CCN2 protein (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	Activity Measured by its binding ability in a functional ELISA. Immobilized Rhesus macaque CTGF at 1 μg/ml can bind Anti-CTGF recombinant antibody (CSB-RA006147MA2HU). The EC₅₀ is 0.6886-0.7825 ng/mL. Biological Activity Assay (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Macaca mulatta CCN family member 2 (CCN2) (Active)

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