Recombinant Mesocricetus auratus Cytochrome P450 2E1 (CYP2E1)

Code CSB-YP006425MRG
MSDS
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Source Yeast
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Code CSB-EP006425MRG
MSDS
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Source E.coli
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Code CSB-EP006425MRG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006425MRG
MSDS
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Source Baculovirus
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Code CSB-MP006425MRG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP2E1
Uniprot No.
Alternative Names
CYP2E1; CYP2ECytochrome P450 2E1; EC 1.14.14.1; 4-nitrophenol 2-hydroxylase; EC 1.14.13.n7; CYPIIE1; Cytochrome P450-J
Species
Mesocricetus auratus (Golden hamster)
Expression Region
2-493
Target Protein Sequence
AVFGVTIAL LVWVATLLIV SIWKQIYSSW NLPPGPFPLP ILGNIFQLDL KNIPKSLTKL AERFGPVFTL HLGSKRIVVL HGYKAVKEVL LNHKNEFSGR GDIPVFQEYM NKGIIFNNGP TWKDVRRFSL SILRDYGMGK QGNEARIQRE AHFLMEELKK TNGQPFDPTF LVGCAPFNVI SDILFHKRFD YNDKTCLRLM SLFNENFYLL STPWIQAYNN FENYLRYLPG SHRKIMKNAS EIRQYTLAKA KEHLQSLDSS CPRDVTDCLL IEMEKEKDSQ EPMYTMENIS VTLADLFFAG TETTSTTLRY GLLILMKYPE VEEKLHEEID RVIGPSRVPV FKDRLEMPYM DAVVHEIQRF ISLIPSNLPH EATRDTMFRG YVIPKGTVVI PTLDSLLYDS QEFPDPEKFK PEHFLNENGK FKYSDHFKAF SAGKRVCVGE GLARMELFLL LTAILQHFNL KSLVDPKDID LNPVTIGFGC VPPEFKLCVI PRS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
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