Recombinant Mitogen-activated protein kinase pmk-3 (pmk-3)

Code CSB-YP529816CXY
MSDS
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Source Yeast
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Code CSB-EP529816CXY
MSDS
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Source E.coli
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Code CSB-EP529816CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP529816CXY
MSDS
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Source Baculovirus
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Code CSB-MP529816CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pmk-3
Uniprot No.
Alternative Names
pmk-3; F42G8.4; Mitogen-activated protein kinase pmk-3; EC 2.7.11.24; Stress-activated protein kinase pmk-3; p38 MAP kinase 3
Species
Caenorhabditis elegans
Expression Region
1-474
Target Protein Sequence
MASVPSSSSL PVSHVRRHED VSTPSAPPTK RSNNQSQPPE SYEPNTWLQQ QREQEQQKKL AAENIKKQSI EATGNNEMVG EEEEDILSKP CGPHKRRFQF VMIRNITFAI PEGYDVEPNS IEYLGGGSFG NVIKTSAVCR DGLRRYVAIK KMREPFFDPH HARRIFRETK LLQLMRHDNI ICALDIYTPD EENDFRDVYV VTEFAGRSLY QILKQQRDYG RRVLTDEHIK FIIYQIIRAL KYIHSANIIH RDLKPGNLAL TDDSDLMILD FGLARSLEKK DTSLTQYVQT RWYRSPEVIY WKIDSYTNLA DMWSLGCIAA ELLTGEPLFP GDEPNAQYQR ITQLCGSPDE ELLTKIENDN SSAIKAVIQS YTTHKRRNFR DVFSAHNPSE DFIDLLEKLL VLDPEKRITV EEAIQHPYLA EFSLPEDEPR ADHIFDLDDS QARTRFEWRD AVWKEIMNYK RLSSSPLIPG EADR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Involved in axon regeneration after injury, probably downstream of dlk-1 and mkk-4 and upstream of mak-2. May phosphorylate mak-2. Plays a role in cilium length regulation, possibly by reducing rab-5 mediated endocytosis. Plays a role in the formation of muscle connections, also called muscle arm extensions, between the body wall and the motor axons in the dorsal and ventral cord.
Subcellular Location
Nucleus. Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell projection, cilium.
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Tissue Specificity
Expressed throughout the intestine.
Database Links

KEGG: cel:CELE_F42G8.4

STRING: 6239.F42G8.4.1

UniGene: Cel.12533

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