Recombinant Mouse ATP-dependent RNA helicase DDX3Y (Ddx3y)

Code CSB-YP717250MO
MSDS
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Source Yeast
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Code CSB-EP717250MO
MSDS
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Source E.coli
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Code CSB-EP717250MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP717250MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP717250MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ddx3y
Uniprot No.
Alternative Names
Ddx3y; D1Pas1-rs1; Dead2ATP-dependent RNA helicase DDX3Y; EC 3.6.4.13; D1Pas1-related sequence 1; DEAD box protein 3; Y-chromosomal; DEAD-box RNA helicase DEAD2; mDEAD2
Species
Mus musculus (Mouse)
Expression Region
2-658
Target Protein Sequence
SQVAAESTA GLDQQFVGLD LKSSDNQNGG GNTESKGRYI PPHLRNRETS KGVCDKDSSG WSCSKDKDAY SSFGSRDSRG KPNYFSDRGS GSRGRFDDHG RNDYDGIGGR DRTGFGKFER SGHSRWSDRS DEDDWSKPLP PSERLEQELF SGGNTGINFE KYDDIPVEAT GNNCPPHIEN FSDIEMGEII MGNIELTRYT RPTPVQKHAI PIIKEKRDLM ACAQTGSGKT AAFLLPILSQ IYTDGPGEAL KAMKENGRYG RRKQYPISLV LAPTRELAVQ IYEEARKFSY RSRVRPCVVY GGADTVQQIR DLERGCHLLV ATPGRLVDMM ERGKIGLDFC KYLVLDEADR MLDMGFEPQI RRIVEQDTMP PKGVRHTMMF SATFPKEIQM LARDFLDEYI FLAVGRVGST SENITQKVVW VEELDKRSFL LDLLNATGKD SLTLVFVETK KGADSLENFL FQERYACTSI HGDRSQKDRE EALHQFRSGR KPILVATAVA ARGLDISNVK HVINFDLPSD IEEYVHRIGR TGRVGNLGLA TSFFNERNLN ITKDLLDLLV EAKQEVPSWL ESMAYEHHYK GSSRGRSKSR FSGGFGARDY RQSSGSANAG FNSNRANSSR SSGSSHNRGF GGGGYGGFYN NDGYGGNYNS QAVDWWGN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable ATP-dependent RNA helicase. During immune response, may enhance IFNB1 expression via IRF3/IRF7 pathway.
Gene References into Functions
  1. Dby mRNA is selectively retained in capacitated mouse spermatozoa. It has an important role in the early zygotic development of male mouse zygotes. This might imply that spermatozoa mRNA is involved in early zygotic and embryonic stages of reproduction. PMID: 20543856
  2. unlike DBY in humans, the role of Dby in spermatogenesis is less obvious in the mouse and its biologic activity may be replaced by that of Ddx3 and D1Pas1. PMID: 16728723
Subcellular Location
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm in an XPO1-dependent manner.
Protein Families
DEAD box helicase family, DDX3/DED1 subfamily
Tissue Specificity
Found in heart, brain, liver, skeletal muscle, kidney and testis. Low expression detected in lung. In testis, expressed in all types of spermatogenic cells including spermatogonia, spermatocytes, spermatids and somatic Sertoli cells within the seminiferou
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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