Recombinant Mouse Acetoacetyl-CoA synthetase (Aacs)

Code CSB-YP001012MO
MSDS
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Source Yeast
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Code CSB-EP001012MO
MSDS
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Source E.coli
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Code CSB-EP001012MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001012MO
MSDS
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Source Baculovirus
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Code CSB-MP001012MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Aacs
Uniprot No.
Alternative Names
AacsAcetoacetyl-CoA synthetase; EC 6.2.1.16
Species
Mus musculus (Mouse)
Expression Region
1-672
Target Protein Sequence
MSKLARLERE EIMECQVMWE PDSKKDTQMD RFRAAVGTAC GLALGNYNDL YHWSVRSYMD FWAEFWKFSG IVYSRMYDEV VDTSKGIADV PEWFRGSRLN YAENLLRHKE NDRVALYVAR EGREEIVKVT FEELRQQVAL FAAAMRKMGV KKGDRVVGYL PNSAHAVEAM LAAASIGAIW SSTSPDFGVN GVLDRFSQIQ PKLIFSVEAV VYNGKEHGHL EKLQRVVKGL PDLQRVVLIP YVLPREKIDI SKIPNSVFLD DFLASGTGAQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM VHSAGGTLIQ HLKEHMLHGN MTSSDILLYY TTVGWMMWNW MVSALATGAS LVLYDGSPLV PTPNVLWDLV DRIGITILGT GAKWLSVLEE KDMKPVETHN LHTLHTILST GSPLKAQSYE YVYRCIKSSV LLGSISGGTD IISCFMGQNS SIPVYKGEIQ ARNLGMAVEA WDEEGKAVWG ASGELVCTKP IPCQPTHFWN DENGSKYRKA YFSKFPGVWA HGDYCRINPK TGGIIMLGRS DGTLNPNGVR FGSSEIYNIV EAFDEVEDSL CVPQYNRDGE ERVVLFLKMA SGHTFQPDLV KRIRDAIRLG LSARHVPSLI LETRGIPYTL NGKKVEVAVK QVMAGRTVEH RGAFSNPETL DLYRDIPELQ DF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.
Gene References into Functions
  1. These results suggest that Sp1 regulates gene expression of AACS in Neuro-2a cells and ketone body utilization affects the balance of histone acetylation. PMID: 29137983
  2. Cleavage of AACS by legumain is critical for the regulation of enzymatic activity and results in gain-of-function changes. PMID: 27129883
  3. Knockdown of AACS inhibits differentiation of 3T3-L1 adipocytes and suppresses expression of the adipocyte markers, peroxisome proliferator-activated receptor gama and CCAAT/enhancer binding protein alpha. PMID: 23123469
  4. Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP-2 and affects serum cholesterol levels. PMID: 22985732
  5. These results suggest that AACS is regulated by SREBP-2 and involves in the normal development of neurons. PMID: 23000407
  6. AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis. PMID: 18514076

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Subcellular Location
Cytoplasm, cytosol.
Protein Families
ATP-dependent AMP-binding enzyme family
Database Links
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