Recombinant Mouse Achaete-scute homolog 2 (Ascl2)

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Code CSB-EP002200MO
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Ascl2
Uniprot No.
Research Area
Developmental Biology
Alternative Names
Ascl2; Mash2Achaete-scute homolog 2; ASH-2; mASH-2; mASH2
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
1-263aa
Target Protein Sequence
MEAHLDWYGVPGLQEASDACPRESCSSALPEAREGANVHFPPHPVPREHFSCAAPELVAGAQGLNASLMDGGALPRLMPTSSGVAGACAARRRQASPELLRCSRRRRSGATEASSSSAAVARRNERERNRVKLVNLGFQALRQHVPHGGANKKLSKVETLRSAVEYIRALQRLLAEHDAVRAALAGGLLTPATPPSDECAQPSASPASASLSCASTSPSPDRLGCSEPTSPRSAYSSEESSCEGELSPMEQELLDFSSWLGGY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
32.8 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

This recombinant Mouse Ascl2 protein is typically achieved by the manipulation of Ascl2 gene expression in E.coli cells so that it expresses large amounts of a recombinant Ascl2 gene. In order to get enough amount of the Ascl2 protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the Ascl2 protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.

Ascl2, a basic helix-loop-helix (bHLH) transcription factor, is required for diploid multipotent trophoblast progenitors, intestinal stem cells, follicular T-helper cells, as well as during epidermal development and myogenesis. It is expressed in neuronal precursors. Ascl2 is a downstream target of the Wnt signaling pathway and also a cancer stem cell marker. Ascl2 has been reported to promote cell proliferation and migration in colon cancer and contribute to epithelial-mesenchymal transition (EMT) and promote metastasis in gastric cancer (GC).

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Target Background

Function
AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
Gene References into Functions
  1. Ascl2 inhibits myogenic differentiation by targeting MRFs and facilitates the generation of postnatal satellite cells. PMID: 27993983
  2. overexpression of the imprinted Ascl2 gene has considerable consequences for placental development, specifically for the parietal trophoblast giant cell and spongiotrophoblast lineages both of which express pregnancy-related hormones. PMID: 27542691
  3. The ascl2 forms a transcriptional switch that is both Wnt responsive and Wnt dependent to define stem cell identity. PMID: 25620640
  4. Ascl2 directly initiates follicular T-helper cell development PMID: 24463518
  5. TSSC3 plays an important role in the differentiation from trophoblast stem cell to trophoblast progenitors and/or labyrinth trophoblasts through the TSSC3/PI3K/AKT/MASH2 signaling pathway. PMID: 23071113
  6. Elevated expression of Ascl2 in a Wnt signalling dependent manner specifically in the stem cell compartment of the intestine neither increases tumour formation nor diminishes survival in a well established intestinal tumour model, the Apc(min) mouse. PMID: 22138533
  7. Ascl2 knockdown results in tumor growth arrest by miRNA-302b-related inhibition of colon cancer progenitor cells PMID: 22384170
  8. Partial loss of Ascl2 function affects all three layers of the mature placenta and causes intrauterine growth restriction. PMID: 21238448
  9. Mash2 gene is preferentially expressed from the paternal allele. PMID: 7773285
  10. Mash2 is a regulator of Krox24, Mob-1, and CXCR4 expression in cultured Schwann cells and negatively regulates their proliferation; a candidate for missing class B bHLH proteins in peripheral nerves PMID: 12196582
  11. structure of the Mash2 gene and placental Mash2 expression at different gestational ages PMID: 12493693
  12. ascl2 is a putative regulator of proliferation that is overexpressed in intestinal neoplasia PMID: 16568095
  13. The combined results from these gain- and loss-of-function experiments imply that Ascl2 controls intestinal stem cell fate. PMID: 19269367

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Subcellular Location
Nucleus.
Database Links
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