Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Ascl2

Uniprot No.

O35885

Research Area

Developmental Biology

Alternative Names

Ascl2; Mash2Achaete-scute homolog 2; ASH-2; mASH-2; mASH2

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-263aa

Target Protein Sequence

MEAHLDWYGVPGLQEASDACPRESCSSALPEAREGANVHFPPHPVPREHFSCAAPELVAGAQGLNASLMDGGALPRLMPTSSGVAGACAARRRQASPELLRCSRRRRSGATEASSSSAAVARRNERERNRVKLVNLGFQALRQHVPHGGANKKLSKVETLRSAVEYIRALQRLLAEHDAVRAALAGGLLTPATPPSDECAQPSASPASASLSCASTSPSPDRLGCSEPTSPRSAYSSEESSCEGELSPMEQELLDFSSWLGGY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

32.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This recombinant Mouse Ascl2 protein is typically achieved by the manipulation of Ascl2 gene expression in E.coli cells so that it expresses large amounts of a recombinant Ascl2 gene. In order to get enough amount of the Ascl2 protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the Ascl2 protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.

Ascl2, a basic helix-loop-helix (bHLH) transcription factor, is required for diploid multipotent trophoblast progenitors, intestinal stem cells, follicular T-helper cells, as well as during epidermal development and myogenesis. It is expressed in neuronal precursors. Ascl2 is a downstream target of the Wnt signaling pathway and also a cancer stem cell marker. Ascl2 has been reported to promote cell proliferation and migration in colon cancer and contribute to epithelial-mesenchymal transition (EMT) and promote metastasis in gastric cancer (GC).

Target Background

Function

AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.

Gene References into Functions

Ascl2 inhibits myogenic differentiation by targeting MRFs and facilitates the generation of postnatal satellite cells. PMID: 27993983
overexpression of the imprinted Ascl2 gene has considerable consequences for placental development, specifically for the parietal trophoblast giant cell and spongiotrophoblast lineages both of which express pregnancy-related hormones. PMID: 27542691
The ascl2 forms a transcriptional switch that is both Wnt responsive and Wnt dependent to define stem cell identity. PMID: 25620640
Ascl2 directly initiates follicular T-helper cell development PMID: 24463518
TSSC3 plays an important role in the differentiation from trophoblast stem cell to trophoblast progenitors and/or labyrinth trophoblasts through the TSSC3/PI3K/AKT/MASH2 signaling pathway. PMID: 23071113
Elevated expression of Ascl2 in a Wnt signalling dependent manner specifically in the stem cell compartment of the intestine neither increases tumour formation nor diminishes survival in a well established intestinal tumour model, the Apc(min) mouse. PMID: 22138533
Ascl2 knockdown results in tumor growth arrest by miRNA-302b-related inhibition of colon cancer progenitor cells PMID: 22384170
Partial loss of Ascl2 function affects all three layers of the mature placenta and causes intrauterine growth restriction. PMID: 21238448
Mash2 gene is preferentially expressed from the paternal allele. PMID: 7773285
Mash2 is a regulator of Krox24, Mob-1, and CXCR4 expression in cultured Schwann cells and negatively regulates their proliferation; a candidate for missing class B bHLH proteins in peripheral nerves PMID: 12196582
structure of the Mash2 gene and placental Mash2 expression at different gestational ages PMID: 12493693
ascl2 is a putative regulator of proliferation that is overexpressed in intestinal neoplasia PMID: 16568095
The combined results from these gain- and loss-of-function experiments imply that Ascl2 controls intestinal stem cell fate. PMID: 19269367

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Subcellular Location

Nucleus.

Database Links

KEGG: mmu:17173

STRING: 10090.ENSMUSP00000009392

UniGene: Mm.196417

Code	CSB-EP002200MO
Abbreviation	Recombinant Mouse Ascl2 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Achaete-scute homolog 2 (Ascl2)

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