Recombinant Mouse All-trans-retinol 13,14-reductase (Retsat)

Code CSB-YP714526MO
MSDS
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Source Yeast
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Code CSB-EP714526MO
MSDS
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Source E.coli
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Code CSB-EP714526MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP714526MO
MSDS
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Source Baculovirus
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Code CSB-MP714526MO
MSDS
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Source Mammalian cell
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Product Details

Purity
≥85% (SDS-PAGE)
Target Names
Retsat
Uniprot No.
Alternative Names
Retsat; Ppsig; All-trans-retinol 13,14-reductase; EC 1.3.99.23; All-trans-13,14-dihydroretinol saturase; RetSat; PPAR-alpha-regulated and starvation-induced gene protein
Species
Mus musculus (Mouse)
Expression Region
26-609
Target Protein Sequence
ASSPN PFAEDVKRPP EPLVTDKEAR KKVLKQAFSV SRVPEKLDAV VIGSGIGGLA SAAVLAKAGK RVLVLEQHTK AGGCCHTFGE NGLEFDTGIH YIGRMREGNI GRFILDQITE GQLDWAPMAS PFDLMILEGP NGRKEFPMYS GRKEYIQGLK KKFPKEEAVI DKYMELVKVV ARGVSHAVLL KFLPLPLTQL LSKFGLLTRF SPFCRASTQS LAEVLQQLGA SRELQAVLSY IFPTYGVTPS HTAFSLHALL VDHYIQGAYY PRGGSSEIAF HTIPLIQRAG GAVLTRATVQ SVLLDSAGRA CGVSVKKGQE LVNIYCPVVI SNAGMFNTYQ HLLPETVRHL PDVKKQLAMV RPGLSMLSIF ICLKGTKEDL KLQSTNYYVY FDTDMDKAME RYVSMPKEKA PEHIPLLFIA FPSSKDPTWE ERFPDRSTMT ALVPMAFEWF EEWQEEPKGK RGVDYETLKN AFVEASMSVI MKLFPQLEGK VESVTGGSPL TNQYYLAAPR GATYGADHDL ARLHPHAMAS IRAQTPIPNL YLTGQDIFTC GLMGALQGAL LCSSAILKRN LYSDLQALGS KVKAQKKKM
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol. Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers. May play a role in the metabolism of vitamin A. Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP. Required for adipocyte differentiation in a 3T3-L1 cell culture model. This effect seems not to mimic the in vivo situation in which animals show increased adiposity in the absence of RETSAT.
Gene References into Functions
  1. Retsat modulates lipid metabolism and the production of reactive oxygen species. PMID: 28927883
  2. Increased adiposity in the retinol saturase-knockout mouse. PMID: 19940255
  3. RetSat-deficiency is associated with alterations in lipid metabolism and the in vivo adipogenic program. PMID: 19940255
  4. Mouse RetSat catalyzes the stereospecific saturation of the C13-C14 double bond of all-trans-retinol to produce (13R)-all-trans-13,14-dihydroretinol. PMID: 18179220
  5. suggest a novel role for retinol saturase in regulating sensitivity to oxidative stress PMID: 17664141
  6. Here, we report the identification and characterization of Ppsig (for PPARalpha-regulated and starvation-induced gene) with unknown biological function from mouse liver PMID: 18289917
  7. RetSat plays an important role in the biology of adipocytes, where it favors normal differentiation, yet is reduced in the obese state. PMID: 19139408

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Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein.
Protein Families
Carotenoid/retinoid oxidoreductase family, CrtISO subfamily
Tissue Specificity
Predominantly expressed in the liver (at protein level). Also expressed at high levels in kidney, intestine, and white fat and brown fat. Weakly expressed in heart, skeletal muscle and testis and barely detected in the lung, brain and spleen. Up-regulated
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
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