Recombinant Mouse Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (St8sia1), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

St8sia1

Uniprot No.

Alternative Names

St8sia1; Siat8; Siat8a; Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase; EC 2.4.99.8; Alpha-2,8-sialyltransferase 8A; Ganglioside GD3 synthase; Ganglioside GT3 synthase; Sialyltransferase 8A; SIAT8-A; Sialyltransferase St8Sia I; ST8SiaI

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Catalyzes the addition of sialic acid in alpha 2,8-linkage to the sialic acid moiety of the ganglioside GM3 to form ganglioside GD3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid. Can catalyze the addition of a second alpha-2,8- sialic acid to GD3 to form GT3. Can use GM1b, GD1a and GT1b as acceptor substrates to synthesize GD1c, GT1a and GQ1b respectively.

Gene References into Functions

Targeted deletion of GD3S protects substantia nigra degeneration and prevents motor deficits associated with MPTP-poisoning. PMID: 28239983
leptin/ObRb-mediated signals were enhanced in hypothalamus of GD3S KO mice due to increased a-series gangliosides, leading to the apparently similar features of energy expenditure between the KO and wild type mice PMID: 27644882
role of GD3 synthase in sciatic nerve and neuronal morphology during peripheral nerve regeneration PMID: 25330147
This study demonistrated that Ganglioside deficiency(B4galnt1 and ST8SIA1 knockout mice causes inflammation and neurodegeneration via the activation of complement system in the spinal cord. PMID: 24673754
Interaction of ganglioside GD3 with an EGF receptor sustains the self-renewal ability of mouse neural stem cells in vitro. PMID: 24198336
The results of this study indicated that destruction of glycolipid-enriched microdomain is caused by ganglioside deficiency with gradual intensity depending on the degree of defects of their compositions. PMID: 21214571
This study showed that NMJs of old mice lacking GD3-synthase (expressing only O- and a-series gangliosides), which do not show an overt neurological phenotype but may develop subclinical synaptic deficits PMID: 19233512
GD3 has a role in hypoxia susceptibility by disabling the c-Src/NF-kappaB survival pathway resulting in lower Mn-SOD expression PMID: 19956670
Molecular cloning and expression and sialylation of O-glycans PMID: 11980897
alpha2,8-sialyltransferase has roles in cell proliferation and mobility PMID: 15645117
The results of this study indicate that synaptic transmission at the NMJ is not crucially dependent on the particular presence of most ganglioside family members. PMID: 18801416

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Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein.

Protein Families

Glycosyltransferase 29 family

Database Links

KEGG: mmu:20449

STRING: 10090.ENSMUSP00000032421

UniGene: Mm.260838

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Recombinant Mouse Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase (St8sia1), partial

Product Details

Customer Reviews and Q&A

Target Background

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