Recombinant Mouse Amyloid beta A4 precursor protein-binding family B member 1-interacting protein (Apbb1ip)

Code CSB-YP844466MO
MSDS
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Source Yeast
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Code CSB-EP844466MO
MSDS
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Source E.coli
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Code CSB-EP844466MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP844466MO
MSDS
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Source Baculovirus
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Code CSB-MP844466MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Apbb1ip
Uniprot No.
Alternative Names
Apbb1ip; Prel1Amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; Proline-rich EVH1 ligand 1; PREL-1; Proline-rich protein 48
Species
Mus musculus (Mouse)
Expression Region
1-670
Target Protein Sequence
MGESNEDIDQ MFSTLLGEMD LLTQSLGVDT LPPPDPNPPR EEFNYTVGFK DLNESLNALE DQDLDALMAD LVADISEAEQ RTIQAQKESS QNQDRFALLR ASDGQGTASG GYGASAAAID VSHHEEALPP PPVEPMLDLL PPPPPPPPPE LLSKEEEEAK AKADKIKLAL EKLKEAKVKK LVVKVHMDDS STKSLMVDER QLARDVLDNL FEKTHCDCNV DWCLYEIYPE LQIERVFEDH ENVVEVLSDW TRDTENKVLF LEKEERYAVF KNPQNFYLDN KGKKENKETN EKMNAKNKEY LLEESFCGTS IIVPELEGAL YLKEDGKKSW KRRYFLLRAS GIYYVPKGKT KTSRDLACFI QFENVNIYYG IQCKMKYKAP TDHCFVLKHP QIQKESQYIK YLCCDDARTL SQWVMGIRIA KYGKTLYDNY QRAVARAGLA SRWTNLGTVG TPMPAQPSTV SSGLKTGTSQ PNGQMPQAIP SAGPPLQEAQ TQIETTKDEK QGLGNHSPGA TRENHRPKSS LPPPPPPVRR SSDTCGSPAL PSKVKGPGTC TFPHPPENFL PPPPPPPPEE DNSGLLPPPP PPPYLEEPPD FVPPPPPPAA VEDSALPPPP PPPPCLSQEI TKSSPLPPKK PLVPPKRQEN QGLPGAPGNS EQDFMSDLMK ALQKKRGNIP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion.
Gene References into Functions
  1. These data identify the requirement of RIAM for formation of immunological synapses and in resulting T cell functions in autoimmunity. PMID: 28348273
  2. we show that leukocyte integrin activation critically depends on RIAM both in vitro and in vivo PMID: 26337492
  3. These in vivo results confirm a role for RIAM in the regulation of some, but not all, leukocyte integrins and suggest that RIAM-regulated integrin activation is required for trafficking of lymphocytes PMID: 26324702
  4. crystal structure of an active, GTP-bound GTPase domain of Rap1 in complex with the Ras association (RA)-pleckstrin homology (PH) structural module of RIAM at 1.65 A, is reported. PMID: 24287201
  5. generated RIAM-null mice and found that they are viable, fertile, and apparently healthy PMID: 25336629

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Subcellular Location
Cell membrane; Peripheral membrane protein. Cell projection, lamellipodium. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Note=Colocalizes with ENA/VASP proteins at lamellipodia tips and focal adhesions, and F-actin at the leading edge. At the membrane surface, associates, via the PH domain, preferentially with the inositol phosphates, PtdIns(5)P and PtdIns(3)P. This binding appears to be necessary for the efficient interaction of the RA domain to Ras-GTPases.
Protein Families
MRL family
Tissue Specificity
Ubiquitously expressed with high expression in the hematopoietic system.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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