Recombinant Mouse Antiviral innate immune response effector IFIT1 (Ifit1)

In Stock
Code CSB-EP011018MO
Abbreviation Recombinant Mouse Ifit1 protein
MSDS
Size $256
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Target Names
Ifit1
Uniprot No.
Research Area
Immunology
Alternative Names
Glucocorticoid-attenuated response gene 16 protein;Interferon-induced 56 kDa protein;Interferon-induced protein with tetratricopeptide repeats 1
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
1-463aa
Target Protein Sequence
MGENADGDQVMENLLQLRCHFTWKLLFENNDIPDLEVRISEQVQFLDIKNPLGMHNLLAYVRHLKGQQDEALQSLKEAEALIQSEQLSKRSLATWGNCAWLHYHRGSLAEAQIYLDKVEKVCKEFSSPFRYRLECAEMDCEEGWALLKCGGGNYKQAMACFAKALKVEPENPEYNTGYAVVAYRQDLDDNFISLEPLRKAVRLNPEDPYLKVLLALKLQDLGEHVEAEAHIEEALSSTSCQSYVIRYAAKYFRRKHRVDKALHLLNRALQASPSSGYLHYQKGLCYKQQISQLRTSRNRQPRRQDNVQELAQQAIHEFQETLKLRPTFEMAYVCMAEVQAEIHQYEEAERNFQKALNNKTLVAHIEQDIHLRYGRFLQFHKQSEDKAITLYLKGLKVEEKSFAWRKLLTALEKVAERRVCQNVHLVESTSLLGLVYKLKGQEKNALFYYEKALRLTGEMNPAF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
60.7 kDa
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism).
Gene References into Functions
  1. ISG20 acts as an indirect regulator of RNA virus replication in the cytoplasm through the upregulation of many other ISGs. PMID: 30232164
  2. IFIT1 expression is associated with podocytes damage, and capable of suppressing some proteins essential to glomerular filtration. PMID: 27823966
  3. Human IFIT1 and mouse IFIT1 have divergent antiviral specificities: only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. PMID: 27240734
  4. Data suggest that, with up-regulation of IFIT1, alphaviruses (Venezuelan equine encephalitis virus; Chikungunya virus) with wild-type 5' untranslated regions are inducers of type I IFN (interferon), suggesting a new mechanism of type I IFN induction. PMID: 25927359
  5. Targeted expression of Ifit1 in hepatocytes protects mice from lipopolysaccharide and D-galactosamine induced lethal hepatitis. PMID: 26459629
  6. IFIT1 is not a dominant restriction factor against three different families of negative-sense RNA viruses. PMID: 26157117
  7. The specificity of IFIT1 for 2'O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2'O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells. PMID: 24098121
  8. Thus, Ifit1 inhibits replication of MTase-defective Japanese encephalitis virus by inhibiting mRNA translation through direct binding to mRNA 5' structures. PMID: 23824812
  9. Study identified Ifit1 as an important innate immune bottleneck. PMID: 22745654
  10. results suggest that virulent strains of West Nile virus have largely evaded the antiviral effects of Ifit1, and viral mutants lacking 2'-O methylation are controlled in vivo by Ifit1-dependent and -independent mechanisms in different cell types PMID: 22589727
  11. IFIT1 bound PPP-RNA antagonizes viruses by sequestering specific viral nucleic acids. PMID: 21642987
  12. results demonstrate that the 2'-O methylation of the 5' cap of viral RNA functions to subvert innate host antiviral responses through escape of IFIT-mediated suppression PMID: 21085181
  13. block different functions of eIF3 by binding to its different subunits. PMID: 15561726
  14. Expression increased significantly during LCMV and WNV infection, being widespread and localized predominantly to common as well as distinct neuronal populations in the brain. PMID: 17079283
  15. Our findings provide the first evidence of a differential pattern of expression of ISG54 and ISG56 genes by IFN-alpha and IFN-beta. PMID: 17553874

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Subcellular Location
Cytoplasm.
Protein Families
IFIT family
Database Links
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