Recombinant Mouse Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1 (Acap1)

Code CSB-YP816986MO
MSDS
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Source Yeast
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Code CSB-EP816986MO
MSDS
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Source E.coli
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Code CSB-EP816986MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP816986MO
MSDS
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Source Baculovirus
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Code CSB-MP816986MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Acap1
Uniprot No.
Alternative Names
Acap1; Centb1; Kiaa0050; Arf-GAP with coiled-coil; ANK repeat and PH domain-containing protein 1; Centaurin-beta-1; Cnt-b1
Species
Mus musculus (Mouse)
Expression Region
1-740
Target Protein Sequence
MTVKLDFEEC LKDSPRFRAS IELVETEVSE LETRLEKLLK LGSCLLESGQ HYLAAGRAFV VGICDLARLG PPEPMMAECL EKFTVSLNHK LDSHAELLDA TQHTLQQQIQ TLVKEGLRGF REARRDFWRG AENLEAALTH NAEVPRRRVQ EAEEAGTALR TARAGYRSRA LDYALQVNVI EDKRKFDIME FVLRLVEAQA TYFQQGHEEL NRLAQYRKEL GTQLHNLVLN SARQKRDMEQ RHVLLKQKEL GGEEPEPSLK EGPSGLVMEG HLFKRASNAF KTWSRRWFTI QNNQLVYQKK YKDPVTVVVD DLRLCTVKLC PDSERRFCFE VVSTSKSCFL QADSERLLQL WVSAVQSSIA SAFSQAHLEN SPRGPGQVSG YHAPGSAATL ACGGAARGRE SGGVGQVAAQ VQSVDGNAQC CDCREPAPEW ASINLGVTLC IQCSGIHRSL GVHFSKVRSL TLDSWEPELV KLMCELGNVI INQIYEARVE AMAVKKPGPS CSRQEKEAWI HAKYVEKKFL TKLPEIRGRR GGRGPPRGHP PVPPKPPIRP HSGIVRSKSE CPSDDMGSLH PGALLFQAAG HPPSLPTMAD ALAHGADVNW VNVGQGNATP LIRATAANSL LACEFLLQNG ANVNQADSAG RGPLHHATIL GHTGLACLFL KRGADLGARD TEGRDPLTIA METTNADIVT LLRLAKMREA EAAQGQAGDE TYLDIFRDFS LMASDDPEKL SRRSHDLHTL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Subcellular Location
Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side.
Database Links
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