Product Details

Purity

>85% (SDS-PAGE)

Target Names

Mapk8ip3

Uniprot No.

Q9ESN9

Alternative Names

Mapk8ip3; Jip3; Jsap1; Syd2C-Jun-amino-terminal kinase-interacting protein 3; JIP-3; JNK-interacting protein 3; JNK MAP kinase scaffold protein 3; JNK/SAPK-associated protein 1; JSAP1; Mitogen-activated protein kinase 8-interacting protein 3; Sunday driver 2

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner. Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation. Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration. Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development. Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation.

Gene References into Functions

JIP3-KO mice after aortic banding exhibited attenuated cardiac function, reduced fibrosis levels and decreased hypertrophic marker proteins. Loss of JIP3 also ameliorated oxidative stress, inflammatory response, apoptosis and endoplasmic reticulum (ER) stress in hearts. PMID: 29604277
JIP3 absence is associated with decreased lipogenesis, oxidative stress and inflammation, supplying a new target for nonalcoholic fatty liver disease treatment. PMID: 29454969
These results establish the critical role of JIP3-dependent axonal lysosome transport in regulating amyloidogenic amyloid precursor protein processing and support a model wherein Abeta production is amplified by plaque-induced axonal lysosome transport defects. PMID: 28784610
Data show that c-Jun NH2-terminal kinase (JNK)-interacting protein-3 (JIP3) participates in the migration of cortical neurons during cortical development by mediating TrkB receptor axonal anterograde transport. PMID: 28259553
JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration PMID: 25571974
Results demonstrated the increased expression of JIP3 in the temporal neocortex of TLE patients and in the experimental model of epileptic seizures PMID: 26002316
These findings reveal that JIP3 regulation of kinesin-1 motility is critical for axon elongation and regeneration. PMID: 25944905
JSAP1 and JLP, which might be paralogous to each other, are critical and functionally redundant in cytokinesis and control ARF6 localization to the midbody by forming a tripartite complex of JSAP1/JLP, active ARF6, and kinesin-1. PMID: 25130574
JIP3 regulates neuronal axon elongation in a kinesin- and JNK-dependent manner PMID: 23576431
Results suggest that JSAP1 is required for the pathfinding of the developing telencephalic commissures in the early brains. PMID: 21685723
syd activates kinesin heavy chain for transport & enhances its motility, increasing its velocity & run length. syd mutants binding KHC but not kinesin light chain are transported to axons & dendrites normally. PMID: 21750526
The results provide evidence that ARF6/JIP3 signaling regulates neurite morphogenesis. PMID: 20493856
Results suggest that endocytic sunday driver (syd) vesicles function in part to carry injury signals back to the cell body, whereas anterograde syd vesicles may play a role in axonal outgrowth and guidance. PMID: 19801628
Jip3 gene disruption causes severe defects in the morphogenesis of the telencephalon. PMID: 12897243
JSAP1 is required for early embryonic neurogenesis PMID: 12968026
form activated by platelet-derived growth factor receptor-alpha is needed for platelet-derived growth factor induced p21WAF1/CIP1 promoter activity independent of p53 PMID: 14506245
c-jun amino-terminal kinase has a role in regulation of the neural differentiation of P19 cells PMID: 15218018
JSAP1 is required for the axon guidance of the telencephalic commissures and the distribution of cellular protein(s) along axons in vivo. PMID: 15572149
Together, these results suggest that JSAP1 may be important for the differentiation of the mesodermal lineages, functioning as a positive factor for cardiomyocyte differentiation, and as an inhibitory factor for differentiation into other lineages. PMID: 16253209
Therefore, the characteristic cellular expression and subcellular distribution of JSAP1 might be beneficial for cells to efficiently link external stimuli to the JNK MAPK pathway and other intracellular machineries. PMID: 16606357
The neonatal death of jsap1-deficient mice is caused by defects in the nervous system. PMID: 17977657
These results suggest that JSAP1 is required for the normal expressions of cytoskeletal and chaperone proteins in the developing lung, and that impaired expressions of these proteins might cause morphogenetic defects observed in jsap1(-/-) lungs. PMID: 18324732
Oxidative stress may induce inflammation in mesangial cells by activating JNK(c-Jun NH(2)-terminal kinase) through either direct activation of JNK or indirectly by suppression of MKP5 activity. PMID: 19776174

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Subcellular Location

Cytoplasm. Golgi apparatus. Cytoplasmic vesicle. Cell projection, growth cone. Cell projection, axon. Cell projection, dendrite. Cytoplasm, perinuclear region.

Protein Families

JIP scaffold family

Tissue Specificity

Highly expressed throughout many regions of the brain and at lower levels in the heart, liver, lung, testes and kidney. All isoforms have been identified in the brain, isoform 1a is also expressed in the spleen and lung.

Database Links

KEGG: mmu:30957

STRING: 10090.ENSMUSP00000085683

UniGene: Mm.43081

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Recombinant Mouse C-Jun-amino-terminal kinase-interacting protein 3 (Mapk8ip3), partial

Product Details

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Target Background

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